Pseudomonas aeruginosa PAO1, PA2800 (vacJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2800
Name
vacJ
Synonym: vacJ
Replicon chromosome
Genomic location 3156860 - 3157564 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251490.1
GI 15597996
Affymetrix PA2800_at
DNASU PaCD00007215
Entrez 879842
GenBank AAG06188.1
INSDC AAG06188.1
NCBI Locus Tag PA2800
protein_id(GenBank) gb|AAG06188.1|AE004707_7|gnl|PseudoCAP|PA2800
TIGR NTL03PA02800
UniParc UPI00000C5812
UniProtKB Acc Q9I043
UniProtKB ID Q9I043_PSEAE
UniRef100 UniRef100_W0YTS4
UniRef50 UniRef50_A0A071L4Q1
UniRef90 UniRef90_A0A071L4Q1

Product

Feature Type CDS
Coding Frame 1
Product Name
VacJ
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (26.15 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.32
Kyte-Doolittle Hydrophobicity Value -0.226
Molecular Weight (kDa) 26.1
Isoelectric Point (pI) 5.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 194 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002769 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2800
Search term: vacJ
Search term: VacJ

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification and characterization of a chromosomal virulence gene, vacJ, required for intercellular spreading of Shigella flexneri.
Suzuki T, Murai T, Fukuda I, Tobe T, Yoshikawa M, Sasakawa C
Mol Microbiol 1994 Jan;11(1):31-41
PubMed ID: 8145644
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
PA2800 plays an important role in both antibiotic susceptibility and virulence in Pseudomonas aeruginosa.
Shen L, Gao X, Wei J, Chen L, Zhao X, Li B, Duan K
Curr Microbiol 2012 Nov;65(5):601-9
PubMed ID: 22878555