Pseudomonas aeruginosa PAO1, PA2851 (efp)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2851
Name
efp
Replicon chromosome
Genomic location 3204514 - 3205080 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251541.1
GI 15598047
Affymetrix PA2851_efp_at
Entrez 882605
GenBank AAG06239.1
INSDC AAG06239.1
NCBI Locus Tag PA2851
protein_id(GenBank) gb|AAG06239.1|AE004711_11|gnl|PseudoCAP|PA2851
TIGR NTL03PA02851
UniParc UPI00000C5843
UniProtKB Acc Q9HZZ2
UniProtKB ID EFP_PSEAE
UniRef100 UniRef100_A6V3N9
UniRef50 UniRef50_Q8R5X5
UniRef90 UniRef90_A6V3N9

Product

Feature Type CDS
Coding Frame 1
Product Name
translation elongation factor P
Product Name Confidence: Class 2
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.06
Kyte-Doolittle Hydrophobicity Value -0.283
Molecular Weight (kDa) 21.0
Isoelectric Point (pI) 4.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3OYY TRANSLATION 09/24/10 Structure of Pseudomonas aeruginosa elongation factor P PSEUDOMONAS AERUGINOSA 1.75 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002717 (565 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2851
Search term: efp

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 45548
Genomic Position 3205016
Position in ORF 503(567)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 35703
Genomic Position 3204704
Position in ORF 191(567)
Reference 14617778

Transposon Insertions in Orthologs

References

Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis.
Aoki H, Adams SL, Chung DG, Yaguchi M, Chuang SE, Ganoza MC.
Nucleic Acids Res. 1991 Nov 25;19(22):6215-20.
PubMed ID: 1956781
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB.
Front Microbiol. 2011;2:162.
PubMed ID: 21863142
PubMed ID: 23239623
PubMed ID: 26384033