Pseudomonas aeruginosa PAO1, PA2851 (efp)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2851
Name
efp
Replicon chromosome
Genomic location 3204514 - 3205080 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251541.1
GI 15598047
Affymetrix PA2851_efp_at
Entrez 882605
GenBank AAG06239.1
INSDC AAG06239.1
NCBI Locus Tag PA2851
protein_id(GenBank) gb|AAG06239.1|AE004711_11|gnl|PseudoCAP|PA2851
TIGR NTL03PA02851
UniParc UPI00000C5843
UniProtKB Acc Q9HZZ2
UniProtKB ID EFP_PSEAE
UniRef100 UniRef100_A6V3N9
UniRef50 UniRef50_Q8R5X5
UniRef90 UniRef90_A6V3N9

Product

Feature Type CDS
Coding Frame 1
Product Name
translation elongation factor P
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.06
Kyte-Doolittle Hydrophobicity Value -0.283
Molecular Weight (kDa) 21.0
Isoelectric Point (pI) 4.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6J7M TRANSFERASE 01/18/19 Complex structure of the Pseudomonas aeruginosa rhamnosyltransferase EarP with the acceptor elongation factor EF-P Pseudomonas aeruginosa PAO1 2.301 X-RAY DIFFRACTION 100.0
3OYY TRANSLATION 09/24/10 Structure of Pseudomonas aeruginosa elongation factor P Pseudomonas aeruginosa 1.75 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002717 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2851
Search term: efp

Human Homologs

References

Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis.
Aoki H, Adams SL, Chung DG, Yaguchi M, Chuang SE, Ganoza MC
Nucleic Acids Res 1991 Nov 25;19(22):6215-20
PubMed ID: 1956781
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142