Pseudomonas aeruginosa PAO1, PA2931 (cifR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2931
Name
cifR
Replicon chromosome
Genomic location 3286624 - 3287214 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 19 transposon mutants in orthologs

Cross-References

RefSeq NP_251621.1
GI 15598127
Affymetrix PA2931_at
DNASU PaCD00007277
Entrez 878998
GenBank AAG06319.1
INSDC AAG06319.1
NCBI Locus Tag PA2931
protein_id(GenBank) gb|AAG06319.1|AE004719_5|gnl|PseudoCAP|PA2931
TIGR NTL03PA02931
UniParc UPI00000C588E
UniProtKB Acc Q9HZR6
UniProtKB ID Q9HZR6_PSEAE
UniRef100 UniRef100_Q9HZR6
UniRef50 UniRef50_Q9HZR6
UniRef90 UniRef90_Q9HZR6

Product

Feature Type CDS
Coding Frame 1
Product Name
CifR
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -1.61
Kyte-Doolittle Hydrophobicity Value -0.189
Molecular Weight (kDa) 21.7
Isoelectric Point (pI) 5.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6NSR TRANSCRIPTION/DNA 01/25/19 TetR family transcriptional regulator CifR C99T-C181R cysteine mutant complexed with 26bp double-strand operator DNA and apo-CifR C99T-C181R Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3 X-RAY DIFFRACTION 98.5
6NSM TRANSCRIPTION/DNA 01/25/19 TetR family transcriptional regulator CifR C99T-C107S-C181R Cysteines mutant complexed with 26bp double-strand operator DNA Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2.8 X-RAY DIFFRACTION 98.0
6NSN TRANSCRIPTION/DNA 01/25/19 TetR family transcriptional regulator CifR C99T-C181R Cysteines mutant complexed with 26bp double-strand operator DNA Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2.6 X-RAY DIFFRACTION 98.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 231 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000241 (6008 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2931
Search term: cifR
Search term: CifR

Human Homologs

References

Cif is negatively regulated by the TetR family repressor CifR.
MacEachran DP, Stanton BA, O'Toole GA
Infect Immun 2008 Jul;76(7):3197-206
PubMed ID: 18458065