Pseudomonas aeruginosa PAO1, PA2944 (cobN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2944
Name
cobN
Replicon chromosome
Genomic location 3300615 - 3304361 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251634.1
GI 15598140
Affymetrix PA2944_cobN_at
Entrez 882831
GenBank AAG06332.1
INSDC AAG06332.1
NCBI Locus Tag PA2944
protein_id(GenBank) gb|AAG06332.1|AE004720_8|gnl|PseudoCAP|PA2944
TIGR NTL03PA02944
UniParc UPI00000C589B
UniProtKB Acc Q9HZQ3
UniProtKB ID COBN_PSEAE
UniRef100 UniRef100_Q9HZQ3
UniRef50 UniRef50_Q9HZQ3
UniRef90 UniRef90_Q9HZQ3

Product

Feature Type CDS
Coding Frame 1
Product Name
cobalamin biosynthetic protein CobN
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -31.19
Kyte-Doolittle Hydrophobicity Value -0.277
Molecular Weight (kDa) 138.5
Isoelectric Point (pI) 5.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 238 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002636 (815 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2944
Search term: cobN

Human Homologs

References

Assay, purification, and characterization of cobaltochelatase, a unique complex enzyme catalyzing cobalt insertion in hydrogenobyrinic acid a,c-diamide during coenzyme B12 biosynthesis in Pseudomonas denitrificans.
Debussche L, Couder M, Thibaut D, Cameron B, Crouzet J, Blanche F
J Bacteriol 1992 Nov;174(22):7445-51
PubMed ID: 1429466