Pseudomonas aeruginosa PAO1, PA2949

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2949
Name
Replicon chromosome
Genomic location 3308391 - 3309338 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251639.1
GI 15598145
Affymetrix PA2949_at
DNASU PaCD00007285
Entrez 882855
GenBank AAG06337.1
INSDC AAG06337.1
NCBI Locus Tag PA2949
protein_id(GenBank) gb|AAG06337.1|AE004721_5|gnl|PseudoCAP|PA2949
TIGR NTL03PA02949
UniParc UPI00000C4EC6
UniProtKB Acc G3XCU7
UniProtKB ID G3XCU7_PSEAE
UniRef100 UniRef100_Q9KJG6
UniRef50 UniRef50_F6AH52
UniRef90 UniRef90_Q9KJG6

Product

Feature Type CDS
Coding Frame 1
Product Name
esterase
Product Name Confidence: Class 3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.10
Kyte-Doolittle Hydrophobicity Value -0.029
Molecular Weight (kDa) 34.8
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 503 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000798 (2785 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2949
Search term: esterase

Human Homologs

Ensembl 86, assembly GRCh38.p7
abhydrolase domain containing 6 [Source:HGNC Symbol;Acc:HGNC:21398]
E-value: 1.2e-33
Percent Identity: 28.1

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 21691
Genomic Position 3308675
Position in ORF 285(948)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 51339
Genomic Position 3308536
Position in ORF 146(948)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_25910
Mutant ID PA14NR: 25817
Genomic Position 2260643
Position in ORF 621(948)
Reference 16477005

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence of the lipase gene lip3 from the antarctic psychotroph Moraxella TA144.
Feller G, Thiry M, Gerday C
Biochim. Biophys. Acta 1991 Feb;1088(2):323-4
PubMed ID: 2001407
A membrane-bound esterase PA2949 from Pseudomonas aeruginosa is expressed and purified from Escherichia coli.
Kovacic F, Bleffert F, Caliskan M, Wilhelm S, Granzin J, Batra-Safferling R, Jaeger KE
FEBS Open Bio 2016 May;6(5):484-93
PubMed ID: 27419054