Pseudomonas aeruginosa PAO1, PA2967 (fabG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2967
Name
fabG
Replicon chromosome
Genomic location 3325379 - 3326122 (- strand)

Cross-References

RefSeq NP_251657.1
GI 15598163
Affymetrix PA2967_fabG_at
DNASU PaCD00007294
Entrez 880433
GenBank AAG06355.1
INSDC AAG06355.1
NCBI Locus Tag PA2967
protein_id(GenBank) gb|AAG06355.1|AE004722_11|gnl|PseudoCAP|PA2967
TIGR NTL03PA02967
UniParc UPI000012A462
UniProtKB Acc O54438
UniProtKB ID FABG_PSEAE
UniRef100 UniRef100_O54438
UniRef50 UniRef50_P0AEK2
UniRef90 UniRef90_O54438

Product

Feature Type CDS
Coding Frame 1
Product Name
3-oxoacyl-[acyl-carrier-protein] reductase
Product Name Confidence: Class 1
Synonyms FabG protein
beta-ketoacyl acyl carrier protein reductase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.52
Kyte-Doolittle Hydrophobicity Value 0.176
Molecular Weight (kDa) 25.6
Isoelectric Point (pI) 6.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Periplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization
Detetected in a periplasmic protein preparation by spheroplasting by osmotic shock.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4BO0 OXIDOREDUCTASE 05/17/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 1-(4-methoxy-1- methylindazol-3-yl)-3-(2-methoxyphenyl)urea at 2.4A resolution PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0
4BO1 OXIDOREDUCTASE 05/18/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with N-(4-chloro-2,5- dimethoxyphenyl)quinoline-8-carboxamide at 2.2A resolution PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 100.0
4BNZ OXIDOREDUCTASE 05/17/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 1-methyl-N-phenylindole- 3-carboxamide at 2.5A resolution PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 100.0
4BO9 OXIDOREDUCTASE 05/18/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 5-(2-(furan-2-ylmethoxy) phenyl)-2-phenyltetrazole at 2.9A resolution PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0
4BO7 OXIDOREDUCTASE 05/18/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with N-(2,3-dihydro-1H-inden- 5-yl)tetrazolo(1,5-b)pyridazin-6-amine at 2.6A resolution PSEUDOMONAS AERUGINOSA 2.6 X-RAY DIFFRACTION 100.0
4BO2 OXIDOREDUCTASE 05/18/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 1-(1-ethylbenzimidazol-2- yl)-3-(2-methoxyphenyl)urea at 1.9A resolution PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 100.0
4BO6 OXIDOREDUCTASE 05/18/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 2,3-dihydroindol-1-yl-(2- thiophen-3-yl-1,3-thiazol-4-yl)methanone at 2.8A resolution PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 100.0
4BO3 OXIDOREDUCTASE 05/18/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 2-(3-(trifluoromethyl) anilino)pyridine-3-sulfonamide at 2.5A resolution PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 100.0
4BO8 OXIDOREDUCTASE 05/18/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 1-(2-amino-4- phenylimidazol-1-yl)-3-(2-fluorophenyl)urea at 2.7A resolution PSEUDOMONAS AERUGINOSA 2.7 X-RAY DIFFRACTION 100.0
4BNY OXIDOREDUCTASE 05/17/13 Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 4-(2-phenylthieno(3,2-d) pyrimidin-4-yl)morpholine at 1.8A resolution PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 652 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002615 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2967
Search term: fabG

Human Homologs

Ensembl 110, assembly GRCh38.p14
carbonyl reductase 4 [Source:HGNC Symbol;Acc:HGNC:25891]
E-value: 4.4e-41
Percent Identity: 42.7

References

The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes.
Rawlings M, Cronan JE Jr
J Biol Chem 1992 Mar 25;267(9):5751-4
PubMed ID: 1556094
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Beta-ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths.
Hoang TT, Sullivan SA, Cusick JK, Schweizer HP
Microbiology (Reading) 2002 Dec;148(Pt 12):3849-3856
PubMed ID: 12480888
Characterization of 3-Oxacyl-Acyl Carrier Protein Reductase Homolog Genes in Pseudomonas aeruginosa PAO1.
Guo QQ, Zhang WB, Zhang C, Song YL, Liao YL, Ma JC, Yu YH, Wang HH
Front Microbiol 2019;10:1028
PubMed ID: 31231314