Pseudomonas aeruginosa PAO1, PA3001

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3001
Name
Replicon chromosome
Genomic location 3359269 - 3360654 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251691.1
GI 15598197
Affymetrix PA3001_at
Entrez 880414
GenBank AAG06389.1
INSDC AAG06389.1
NCBI Locus Tag PA3001
protein_id(GenBank) gb|AAG06389.1|AE004725_2|gnl|PseudoCAP|PA3001
TIGR NTL03PA03001
UniParc UPI00000C58BF
UniProtKB Acc Q9HZK4
UniProtKB ID Q9HZK4_PSEAE
UniRef100 UniRef100_W0YRZ4
UniRef50 UniRef50_Q02PG5
UniRef90 UniRef90_Q02PG5

Product

Feature Type CDS
Coding Frame 1
Product Name
probable glyceraldehyde-3-phosphate dehydrogenase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (50.11 kDa, PMID:21751344)
Charge (pH 7) 4.02
Kyte-Doolittle Hydrophobicity Value -0.168
Molecular Weight (kDa) 50.1
Isoelectric Point (pI) 8.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4247
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 607 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002581 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3001

Human Homologs

Ensembl 110, assembly GRCh38.p14
glyceraldehyde-3-phosphate dehydrogenase, spermatogenic [Source:HGNC Symbol;Acc:HGNC:24864]
E-value: 2.2e-46
Percent Identity: 37.6

References

Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicin.
Bibb MJ, Sherman DH, Omura S, Hopwood DA
Gene 1994 May 3;142(1):31-9
PubMed ID: 8181754
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344