Pseudomonas aeruginosa PAO1, PA3044 (rocsS2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3044
Name
rocsS2
Replicon chromosome
Genomic location 3406321 - 3408546 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentOverexpression of rocS2 leads to the production of CupB and CupC fimbriae, as well as repression of the MexAB-OprM efflux pump. RocS2 can interact with three response regulators: RocA2 (PA3045), RocA1 (PA3948) and RocR (PA3947).
CommentFunctional studies in Pseudomonas aeruginosa PAK have shown that RocS2 is part of a signalling network that induces the expression of the cupB and cupC gene clusters, leading to the production and assembly of two distinct types of fimbriae on the cell surface and increases biofilm formation. Expression of rocS2 also represses the expression of the mexAB-oprM gene cluster, leading to increased sensitivity to beta-lactams and other antibiotics. This signalling network involves at least three response regulators: RocA1 (PA3948), RocA2 (PA3045) and RocR (PA3947). Genetic and bacterial two hybrid analyses have shown that RocS2 signals through all three response regulators.

Cross-References

RefSeq NP_251734.1
GI 15598240
Affymetrix PA3044_at
Entrez 882873
GenBank AAG06432.1
INSDC AAG06432.1
NCBI Locus Tag PA3044
protein_id(GenBank) gb|AAG06432.1|AE004729_6|gnl|PseudoCAP|PA3044
TIGR NTL03PA03044
UniParc UPI00000C58E4
UniProtKB Acc Q9HZG4
UniProtKB ID Q9HZG4_PSEAE
UniRef100 UniRef100_Q9HZG4
UniRef50 UniRef50_A6V332
UniRef90 UniRef90_Q9HZG4

Product

Feature Type CDS
Coding Frame 1
Product Name
Two-component sensor RocS2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.22
Kyte-Doolittle Hydrophobicity Value -0.199
Molecular Weight (kDa) 82.2
Isoelectric Point (pI) 7.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002543 (301 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3044
Search term: rocsS2

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes.
Kulasekara HD, Ventre I, Kulasekara BR, Lazdunski A, Filloux A, Lory S
Mol Microbiol 2005 Jan;55(2):368-80
PubMed ID: 15659157
Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression.
Sivaneson M, Mikkelsen H, Ventre I, Bordi C, Filloux A
Mol Microbiol 2011 Mar;79(5):1353-66
PubMed ID: 21205015