Pseudomonas aeruginosa PAO1, PA3064 (pelA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3064
Name
pelA
Replicon chromosome
Genomic location 3431045 - 3433891 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251754.1
GI 15598260
Affymetrix PA3064_at
Entrez 878833
GenBank AAG06452.1
INSDC AAG06452.1
NCBI Locus Tag PA3064
protein_id(GenBank) gb|AAG06452.1|AE004730_16|gnl|PseudoCAP|PA3064
TIGR NTL03PA03064
UniParc UPI00000C58F8
UniProtKB Acc Q9HZE4
UniProtKB ID Q9HZE4_PSEAE
UniRef100 UniRef100_Q9HZE4
UniRef50 UniRef50_Q9HZE4
UniRef90 UniRef90_Q9HZE4

Product

Feature Type CDS
Coding Frame 1
Product Name
PelA
Product Name Confidence: Class 1
Synonyms PelA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 7.73
Kyte-Doolittle Hydrophobicity Value -0.203
Molecular Weight (kDa) 104.9
Isoelectric Point (pI) 8.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5TCB HYDROLASE 09/14/16 Structure of the glycoside hydrolase domain of PelA from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.535 X-RAY DIFFRACTION 100.0
5TSY HYDROLASE 10/31/16 Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.9 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 49 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002525 (338 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3064
Search term: pelA
Search term: PelA

Human Homologs

References

The pel genes of the Pseudomonas aeruginosa PAK strain are involved at early and late stages of biofilm formation.
Vasseur P, Vallet-Gely I, Soscia C, Genin S, Filloux A
Microbiology (Reading) 2005 Mar;151(Pt 3):985-997
PubMed ID: 15758243