Pseudomonas aeruginosa PAO1, PA3068 (gdhB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3068
Name
gdhB
Replicon chromosome
Genomic location 3436578 - 3441440 (- strand)
Transposon Mutants 10 transposon mutants in PAO1
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_251758.1
GI 15598264
Affymetrix PA3068_at
Entrez 878664
GenBank AAG06456.1
INSDC AAG06456.1
NCBI Locus Tag PA3068
protein_id(GenBank) gb|AAG06456.1|AE004731_4|gnl|PseudoCAP|PA3068
TIGR NTL03PA03068
UniParc UPI00000C6EA7
UniProtKB Acc Q9HZE0
UniProtKB ID DHE2_PSEAE
UniRef100 UniRef100_Q9HZE0
UniRef50 UniRef50_Q9HZE0
UniRef90 UniRef90_Q9HZE0

Product

Feature Type CDS
Coding Frame 1
Product Name
NAD-dependent glutamate dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (182.64 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -19.05
Kyte-Doolittle Hydrophobicity Value -0.225
Molecular Weight (kDa) 182.6
Isoelectric Point (pI) 6.14

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 168 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002524 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3068
Search term: gdhB

Human Homologs

References

The gdhB gene of Pseudomonas aeruginosa encodes an arginine-inducible NAD(+)-dependent glutamate dehydrogenase which is subject to allosteric regulation.
Lu CD, Abdelal AT
J Bacteriol 2001 Jan;183(2):490-9
PubMed ID: 11133942
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344