Pseudomonas aeruginosa PAO1, PA3077 (cprR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3077
Name
cprR
Replicon chromosome
Genomic location 3450838 - 3451509 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251767.1
GI 15598273
Affymetrix PA3077_at
DNASU PaCD00007359
Entrez 882974
GenBank AAG06465.1
INSDC AAG06465.1
NCBI Locus Tag PA3077
protein_id(GenBank) gb|AAG06465.1|AE004731_13|gnl|PseudoCAP|PA3077
TIGR NTL03PA03077
UniParc UPI00000C5904
UniProtKB Acc Q9HZD1
UniProtKB ID Q9HZD1_PSEAE
UniRef100 UniRef100_W0YV68
UniRef50 UniRef50_W0YV68
UniRef90 UniRef90_W0YV68

Product

Feature Type CDS
Coding Frame 1
Product Name
CprR
Product Name Confidence: Class 1
Synonyms cationic peptide resistance regulator
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.17
Kyte-Doolittle Hydrophobicity Value -0.142
Molecular Weight (kDa) 24.8
Isoelectric Point (pI) 6.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
cprR cprR is one part of a two-component regulatory system. It with its counterpart cprS induce the Arn operon to confer resistance to peptide antibiotics. [PMID:23006746] ARO:3005063 protein homolog model Perfect 448.7 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 625 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002515 (325 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3077
Search term: cprR
Search term: CprR

Human Homologs

References

A two-component system plays an important role in the root-colonizing ability of Pseudomonas fluorescens strain WCS365.
Dekkers LC, Bloemendaal CJ, de Weger LA, Wijffelman CA, Spaink HP, Lugtenberg BJ
Mol Plant Microbe Interact 1998 Jan;11(1):45-56
PubMed ID: 9425686
The two-component system CprRS senses cationic peptides and triggers adaptive resistance in Pseudomonas aeruginosa independently of ParRS.
Fernández L, Jenssen H, Bains M, Wiegand I, Gooderham WJ, Hancock RE
Antimicrob Agents Chemother 2012 Dec;56(12):6212-22
PubMed ID: 23006746
Polymyxin resistance of Pseudomonas aeruginosa phoQ mutants is dependent on additional two-component regulatory systems.
Gutu AD, Sgambati N, Strasbourger P, Brannon MK, Jacobs MA, Haugen E, Kaul RK, Johansen HK, Høiby N, Moskowitz SM
Antimicrob Agents Chemother 2013 May;57(5):2204-15
PubMed ID: 23459479