Pseudomonas aeruginosa PAO1, PA3078 (cprS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3078
Name
cprS
Replicon chromosome
Genomic location 3451506 - 3452801 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251768.1
GI 15598274
Affymetrix PA3078_at
Entrez 882975
GenBank AAG06466.1
INSDC AAG06466.1
NCBI Locus Tag PA3078
protein_id(GenBank) gb|AAG06466.1|AE004731_14|gnl|PseudoCAP|PA3078
TIGR NTL03PA03078
UniParc UPI00000C5905
UniProtKB Acc Q9HZD0
UniProtKB ID Q9HZD0_PSEAE
UniRef100 UniRef100_W0YS69
UniRef50 UniRef50_A6V2Z9
UniRef90 UniRef90_A6V2Z9

Product

Feature Type CDS
Coding Frame 1
Product Name
CprS
Product Name Confidence: Class 1
Synonyms cationic peptide resistance sensor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -17.60
Kyte-Doolittle Hydrophobicity Value -0.235
Molecular Weight (kDa) 48.4
Isoelectric Point (pI) 4.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
cprS cprS is part of a two-component regulatory system that, with its counterpart cprR, induces the Arn operon in the presence of cationic peptides to confer resistance. [PMID:23006746] ARO:3005064 protein homolog model Perfect 857.4 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 467 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002514 (325 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3078
Search term: cprS
Search term: CprS

Human Homologs

References

A two-component system plays an important role in the root-colonizing ability of Pseudomonas fluorescens strain WCS365.
Dekkers LC, Bloemendaal CJ, de Weger LA, Wijffelman CA, Spaink HP, Lugtenberg BJ
Mol Plant Microbe Interact 1998 Jan;11(1):45-56
PubMed ID: 9425686
The two-component system CprRS senses cationic peptides and triggers adaptive resistance in Pseudomonas aeruginosa independently of ParRS.
Fernández L, Jenssen H, Bains M, Wiegand I, Gooderham WJ, Hancock RE
Antimicrob Agents Chemother 2012 Dec;56(12):6212-22
PubMed ID: 23006746
Polymyxin resistance of Pseudomonas aeruginosa phoQ mutants is dependent on additional two-component regulatory systems.
Gutu AD, Sgambati N, Strasbourger P, Brannon MK, Jacobs MA, Haugen E, Kaul RK, Johansen HK, Høiby N, Moskowitz SM
Antimicrob Agents Chemother 2013 May;57(5):2204-15
PubMed ID: 23459479