Pseudomonas aeruginosa PAO1, PA3115 (fimV)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3115
Name
fimV
Replicon chromosome
Genomic location 3495446 - 3498205 (- strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251805.1
GI 15598311
Affymetrix PA3115_at
Entrez 879911
GenBank AAG06503.1
INSDC AAG06503.1
NCBI Locus Tag PA3115
protein_id(GenBank) gb|AAG06503.1|AE004735_10|gnl|PseudoCAP|PA3115
TIGR NTL03PA03115
UniParc UPI00000C5916
UniProtKB Acc Q9HZA6
UniProtKB ID Q9HZA6_PSEAE
UniRef100 UniRef100_Q9HZA6
UniRef50 UniRef50_Q9HZA6
UniRef90 UniRef90_Q9HZA6

Product

Feature Type CDS
Coding Frame 1
Product Name
Motility protein FimV
Product Name Confidence: Class 1
Synonyms motility protein FimV
Evidence for Translation
Detected using LC-MS/MS (96.93 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -86.16
Kyte-Doolittle Hydrophobicity Value -0.384
Molecular Weight (kDa) 96.9
Isoelectric Point (pI) 3.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4MAL UNKNOWN FUNCTION 08/16/13 TPR3 of FimV from P. aeruginosa (PAO1) Pseudomonas aeruginosa 2.05 X-RAY DIFFRACTION 100.0
4MBQ UNKNOWN FUNCTION 08/19/13 TPR3 of FimV from P. aeruginosa (PAO1) Pseudomonas aeruginosa 2.006 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 96 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002483 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3115
Search term: fimV

Human Homologs

References

Identification of a novel gene, fimV, involved in twitching motility in Pseudomonas aeruginosa.
Semmler ABT, Whitchurch CB, Leech AJ, Mattick JS
Microbiology (Reading) 2000 Jun;146 ( Pt 6):1321-1332
PubMed ID: 10846211
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344