Pseudomonas aeruginosa PAO1, PA3153 (wzx)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3153
Name
wzx
Synonym: wbpF
Synonym: rfbX
Replicon chromosome
Genomic location 3536576 - 3537811 (- strand)

Cross-References

RefSeq NP_251843.1
GI 15598349
Affymetrix PA3153_wzx_at
Entrez 882590
GenBank AAG06541.1
INSDC AAG06541.1
NCBI Locus Tag PA3153
protein_id(GenBank) gb|AAG06541.1|AE004739_3|gnl|PseudoCAP|PA3153
TIGR NTL03PA03153
UniParc UPI00000DA0EF
UniProtKB Acc G3XD19
UniProtKB ID G3XD19_PSEAE
UniRef100 UniRef100_G3XD19
UniRef50 UniRef50_G3XD19
UniRef90 UniRef90_G3XD19

Product

Feature Type CDS
Coding Frame 1
Product Name
O-antigen translocase
Product Name Confidence: Class 1
Synonyms O-antigen flippase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 5.07
Kyte-Doolittle Hydrophobicity Value 0.968
Molecular Weight (kDa) 45.3
Isoelectric Point (pI) 8.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0085
Category LPS O-antigen (P. aeruginosa)
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 76 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005137 (38 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3153
Search term: wzx

Human Homologs

References

An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase.
Liu D, Cole RA, Reeves PR
J Bacteriol 1996 Apr;178(7):2102-7
PubMed ID: 8606190
Effect of wzx (rfbX) mutations on A-band and B-band lipopolysaccharide biosynthesis in Pseudomonas aeruginosa O5.
Burrows LL, Lam JS
J Bacteriol 1999 Feb;181(3):973-80
PubMed ID: 9922263
A cationic lumen in the Wzx flippase mediates anionic O-antigen subunit translocation in Pseudomonas aeruginosa PAO1.
Islam ST, Fieldhouse RJ, Anderson EM, Taylor VL, Keates RA, Ford RC, Lam JS
Mol Microbiol 2012 Jun;84(6):1165-76
PubMed ID: 22554073
A cationic lumen in the Wzx flippase mediates anionic O-antigen subunit translocation in Pseudomonas aeruginosa PAO1.
Islam ST, Fieldhouse RJ, Anderson EM, Taylor VL, Keates RA, Ford RC, Lam JS
Mol Microbiol 2012 Jun;84(6):1165-76
PubMed ID: 22554073