Pseudomonas aeruginosa PAO1, PA3154 (wzy)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3154
Name
wzy
Synonym: rfc
Replicon chromosome
Genomic location 3537808 - 3539124 (- strand)

Cross-References

RefSeq NP_251844.1
GI 15598350
Affymetrix PA3154_wzy_at
Entrez 882652
GenBank AAG06542.1
INSDC AAG06542.1
NCBI Locus Tag PA3154
protein_id(GenBank) gb|AAG06542.1|AE004739_4|gnl|PseudoCAP|PA3154
TIGR NTL03PA03154
UniParc UPI00000DA0D8
UniProtKB Acc G3XCW3
UniProtKB ID G3XCW3_PSEAE
UniRef100 UniRef100_G3XCW3
UniRef50 UniRef50_G3XCW3
UniRef90 UniRef90_G3XCW3

Product

Feature Type CDS
Coding Frame 1
Product Name
B-band O-antigen polymerase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 12.84
Kyte-Doolittle Hydrophobicity Value 0.814
Molecular Weight (kDa) 48.9
Isoelectric Point (pI) 9.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0085
Category LPS O-antigen (P. aeruginosa)
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG008410 (33 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3154
Search term: wzy

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular cloning and characterization of the rfc gene of Pseudomonas aeruginosa (serotype O5).
de Kievit TR, Dasgupta T, Schweizer H, Lam JS
Mol Microbiol 1995 May;16(3):565-74
PubMed ID: 7565115
Dual conserved periplasmic loops possess essential charge characteristics that support a catch-and-release mechanism of O-antigen polymerization by Wzy in Pseudomonas aeruginosa PAO1.
Islam ST, Gold AC, Taylor VL, Anderson EM, Ford RC, Lam JS
J Biol Chem 2011 Jun 10;286(23):20600-5
PubMed ID: 21498511