Pseudomonas aeruginosa PAO1, PA3162 (rpsA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3162
Name
rpsA
Replicon chromosome
Genomic location 3548110 - 3549789 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251852.1
GI 15598358
Affymetrix PA3162_rpsA_at
Entrez 882562
GenBank AAG06550.1
INSDC AAG06550.1
NCBI Locus Tag PA3162
protein_id(GenBank) gb|AAG06550.1|AE004740_3|gnl|PseudoCAP|PA3162
TIGR NTL03PA03162
UniParc UPI00000C5930
UniProtKB Acc Q9HZ71
UniProtKB ID RS1_PSEAE
UniRef100 UniRef100_Q9HZ71
UniRef50 UniRef50_P0AG69
UniRef90 UniRef90_Q9HZ71

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (61.87 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -30.82
Kyte-Doolittle Hydrophobicity Value -0.343
Molecular Weight (kDa) 61.9
Isoelectric Point (pI) 4.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 598 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002453 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3162
Search term: rpsA

References

Ribosomal protein S1 specifically binds to the 5' untranslated region of the Pseudomonas aeruginosa stationary-phase sigma factor rpoS mRNA in the logarithmic phase of growth.
Sevo M, Buratti E, Venturi V
J. Bacteriol. 2004 Aug;186(15):4903-9
PubMed ID: 15262927
Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA.
Schnier J, Kimura M, Foulaki K, Subramanian AR, Isono K, Wittmann-Liebold B
Proc. Natl. Acad. Sci. U.S.A. 1982 Feb;79(4):1008-11
PubMed ID: 7041110
Ribosomal protein S1 is required for translation of most, if not all, natural mRNAs in Escherichia coli in vivo.
Sørensen MA, Fricke J, Pedersen S
J. Mol. Biol. 1998 Jul;280(4):561-9
PubMed ID: 9677288
A probable mixed-function supraoperon in Pseudomonas exhibits gene organization features of both intergenomic conservation and gene shuffling.
Xie G, Bonner CA, Jensen RA
J. Mol. Evol. 1999 Jul;49(1):108-21
PubMed ID: 10368439
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344