Pseudomonas aeruginosa PAO1, PA3168 (gyrA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3168
Name
gyrA
Replicon chromosome
Genomic location 3556427 - 3559198 (- strand)

Cross-References

RefSeq NP_251858.1
GI 15598364
Affymetrix PA3168_gyrA_at
Entrez 882800
GenBank AAG06556.1
INSDC AAG06556.1
NCBI Locus Tag PA3168
protein_id(GenBank) gb|AAG06556.1|AE004741_1|gnl|PseudoCAP|PA3168
TIGR NTL03PA03168
UniParc UPI000012BE96
UniProtKB Acc P48372
UniProtKB ID GYRA_PSEAE
UniRef100 UniRef100_P48372
UniRef50 UniRef50_P0AES4
UniRef90 UniRef90_P48372

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA gyrase subunit A
Product Name Confidence: Class 1
Synonyms DNA topoisomerase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -40.06
Kyte-Doolittle Hydrophobicity Value -0.248
Molecular Weight (kDa) 101.1
Isoelectric Point (pI) 4.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
Mycobacterium tuberculosis gyrA conferring resistance to fluoroquinolones Point mutation of Mycobacterium tuberculosis gyrA resulted in the lowered affinity between fluoroquinolones and gyrA. Thus, conferring resistance. ARO:3003295 protein variant model S95T, A85T Strict 0.0 0.428732 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 631 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002447 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3168
Search term: gyrA

References

Cloning and nucleotide sequence of Pseudomonas aeruginosa DNA gyrase gyrA gene from strain PAO1 and quinolone-resistant clinical isolates.
Kureishi A, Diver JM, Beckthold B, Schollaardt T, Bryan LE
Antimicrob. Agents Chemother. 1994 Sep;38(9):1944-52
PubMed ID: 7811002