Pseudomonas aeruginosa PAO1, PA3175 (hutE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3175
Name
hutE
Replicon chromosome
Genomic location 3565189 - 3566124 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251865.1
GI 15598371
Affymetrix PA3175_at
Entrez 882685
GenBank AAG06563.1
INSDC AAG06563.1
NCBI Locus Tag PA3175
protein_id(GenBank) gb|AAG06563.1|AE004741_8|gnl|PseudoCAP|PA3175
TIGR NTL03PA03175
UniParc UPI00000C5937
UniProtKB Acc Q9HZ59
UniProtKB ID HUTG_PSEAE
UniRef100 UniRef100_Q9HZ59
UniRef50 UniRef50_Q4KJT9
UniRef90 UniRef90_Q9HZ59

Product

Feature Type CDS
Coding Frame 1
Product Name
HutE
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.29
Kyte-Doolittle Hydrophobicity Value -0.249
Molecular Weight (kDa) 34.2
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 264 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002440 (346 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3175
Search term: hutE
Search term: HutE

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 11577
Genomic Position 3565796
Position in ORF 608(936)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 6028
Genomic Position 3565665
Position in ORF 477(936)
Reference 14617778

Transposon Insertions in Orthologs

References

The origin and ecological significance of multiple branches for histidine utilization in Pseudomonas aeruginosa PAO1.
Gerth ML, Ferla MP, Rainey PB
Environ. Microbiol. 2012 Aug;14(8):1929-40
PubMed ID: 22225844