Pseudomonas aeruginosa PAO1, PA3177

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3177
Name
Replicon chromosome
Genomic location 3567606 - 3568529 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 6 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_251867.1
GI 15598373
Affymetrix PA3177_at
DNASU PaCD00007399
Entrez 882661
GenBank AAG06565.1
INSDC AAG06565.1
NCBI Locus Tag PA3177
protein_id(GenBank) gb|AAG06565.1|AE004742_1|gnl|PseudoCAP|PA3177
TIGR NTL03PA03177
UniParc UPI00000C5939
UniProtKB Acc Q9HZ57
UniProtKB ID Q9HZ57_PSEAE
UniRef100 UniRef100_Q9HZ57
UniRef50 UniRef50_A0A0D0T5R0
UniRef90 UniRef90_Q9HZ57

Product

Feature Type CDS
Coding Frame 1
Product Name
diguanylate cyclase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.16
Kyte-Doolittle Hydrophobicity Value -0.436
Molecular Weight (kDa) 34.4
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 456 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000167 (2758 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3177

Human Homologs

References

The PA3177 Gene Encodes an Active Diguanylate Cyclase That Contributes to Biofilm Antimicrobial Tolerance but Not Biofilm Formation by Pseudomonas aeruginosa.
Poudyal B, Sauer K
Antimicrob Agents Chemother 2018 Oct;62(10)
PubMed ID: 30082282