Pseudomonas aeruginosa PAO1, PA3217 (cyaB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3217
Name
cyaB
Replicon chromosome
Genomic location 3605684 - 3607075 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251907.1
GI 15598413
Affymetrix PA3217_at
Entrez 882555
GenBank AAG06605.1
INSDC AAG06605.1
NCBI Locus Tag PA3217
protein_id(GenBank) gb|AAG06605.1|AE004745_2|gnl|PseudoCAP|PA3217
TIGR NTL03PA03217
UniParc UPI00000C5957
UniProtKB Acc Q9HZ23
UniProtKB ID Q9HZ23_PSEAE
UniRef100 UniRef100_Q9HZ23
UniRef50 UniRef50_Q9HZ23
UniRef90 UniRef90_Q9HZ23

Product

Feature Type CDS
Coding Frame 1
Product Name
CyaB
Product Name Confidence: Class 1
Synonyms adenylate cyclase
Evidence for Translation
Charge (pH 7) 12.71
Kyte-Doolittle Hydrophobicity Value 0.124
Molecular Weight (kDa) 51.4
Isoelectric Point (pI) 9.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3R5G LYASE 03/18/11 Crystal structure of the adenylyl cyclase CyaB from P. aeruginosa Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse model of acute pneumonia
Reference 14977975

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 257 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002398 (355 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3217
Search term: cyaB
Search term: CyaB

Human Homologs

Ensembl 110, assembly GRCh38.p14
guanylate cyclase 2F, retinal [Source:HGNC Symbol;Acc:HGNC:4691]
E-value: 6.8e-11
Percent Identity: 27.5

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Isolation and characterization of multiple adenylate cyclase genes from the cyanobacterium Anabaena sp. strain PCC 7120.
Katayama M, Ohmori M
J Bacteriol 1997 Jun;179(11):3588-93
PubMed ID: 9171404