Pseudomonas aeruginosa PAO1, PA3257 (algO)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3257
Name	algO Synonym: tsp Synonym: prc
Replicon	chromosome
Genomic location	3642931 - 3645060 (+ strand)
Transposon Mutants	3 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_251947.1
GI	15598453
Affymetrix	PA3257_prc_at
Entrez	882420
GenBank	AAG06645.1
INSDC	AAG06645.1
NCBI Locus Tag	PA3257
protein_id(GenBank)	gb|AAG06645.1|AE004748_3|gnl|PseudoCAP|PA3257
TIGR	NTL03PA03257
UniParc	UPI00000C597A
UniProtKB Acc	Q9HYY3
UniProtKB ID	Q9HYY3_PSEAE
UniRef100	UniRef100_Q9HYY3
UniRef50	UniRef50_Q4K8E7
UniRef90	UniRef90_Q9HYY3

Product

Feature Type	CDS
Coding Frame	1
Product Name	AlgO Product Name Confidence: Class 1
Synonyms	periplasmic tail-specific protease
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-2.31
Kyte-Doolittle Hydrophobicity Value	-0.503
Molecular Weight (kDa)	78.2
Isoelectric Point (pI)	6.61

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 505 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002353 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3257 Search term: algO Search term: AlgO

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3. Hara H, Yamamoto Y, Higashitani A, Suzuki H, Nishimura Y J Bacteriol 1991 Aug;173(15):4799-813 PubMed ID: 1856173

Tsp: a tail-specific protease that selectively degrades proteins with nonpolar C termini. Silber KR, Keiler KC, Sauer RT Proc Natl Acad Sci U S A 1992 Jan 1;89(1):295-9 PubMed ID: 1729701

Multiple antibiotic susceptibility associated with inactivation of the prc gene. Seoane A, Sabbaj A, McMurry LM, Levy SB J Bacteriol 1992 Dec;174(23):7844-7 PubMed ID: 1447154

Evidence for PDZ domains in bacteria, yeast, and plants. Ponting CP Protein Sci 1997 Feb;6(2):464-8 PubMed ID: 9041651

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

A complex multilevel attack on Pseudomonas aeruginosa algT/U expression and algT/U activity results in the loss of alginate production. Sautter R, Ramos D, Schneper L, Ciofu O, Wassermann T, Koh CL, Heydorn A, Hentzer M, Høiby N, Kharazmi A, Molin S, Devries CA, Ohman DE, Mathee K Gene 2012 May 1;498(2):242-53 PubMed ID: 22088575

Prc protease promotes mucoidy in mucA mutants of Pseudomonas aeruginosa. Reiling SA, Jansen JA, Henley BJ, Singh S, Chattin C, Chandler M, Rowen DW Microbiology (Reading) 2005 Jul;151(Pt 7):2251-2261 PubMed ID: 16000715