Pseudomonas aeruginosa PAO1, PA3296 (phoA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3296
Name
phoA
Replicon chromosome
Genomic location 3689521 - 3690951 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251986.1
GI 15598492
Affymetrix PA3296_phoA_at
Entrez 882459
GenBank AAG06684.1
INSDC AAG06684.1
NCBI Locus Tag PA3296
protein_id(GenBank) gb|AAG06684.1|AE004751_10|gnl|PseudoCAP|PA3296
TIGR NTL03PA03296
UniParc UPI0000131FFC
UniProtKB Acc P35483
UniProtKB ID PPBH_PSEAE
UniRef100 UniRef100_P35483
UniRef50 UniRef50_P00634
UniRef90 UniRef90_P35483

Product

Feature Type CDS
Coding Frame 1
Product Name
alkaline phosphatase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) -0.71
Kyte-Doolittle Hydrophobicity Value -0.329
Molecular Weight (kDa) 50.4
Isoelectric Point (pI) 6.86

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 247 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002320 (298 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3296
Search term: phoA

Human Homologs

Ensembl 110, assembly GRCh38.p14
alkaline phosphatase, biomineralization associated [Source:HGNC Symbol;Acc:HGNC:438]
E-value: 5.7e-29
Percent Identity: 29.2
Ensembl 110, assembly GRCh38.p14
alkaline phosphatase, biomineralization associated [Source:HGNC Symbol;Acc:HGNC:438]
E-value: 5.7e-29
Percent Identity: 29.2

References

Refined structure of alkaline phosphatase from Escherichia coli at 2.8 A resolution.
Sowadski JM, Handschumacher MD, Murthy HM, Foster BA, Wyckoff HW
J Mol Biol 1985 Nov 20;186(2):417-33
PubMed ID: 3910843
The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein.
DuBose RF, Hartl DL
Mol Biol Evol 1990 Nov;7(6):547-77
PubMed ID: 2129542