Pseudomonas aeruginosa PAO1, PA3297

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3297
Name
Synonym: hrpA
Replicon chromosome
Genomic location 3691024 - 3695004 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251987.1
GI 15598493
Affymetrix PA3297_at
Entrez 882460
GenBank AAG06685.1
INSDC AAG06685.1
NCBI Locus Tag PA3297
protein_id(GenBank) gb|AAG06685.1|AE004752_1|gnl|PseudoCAP|PA3297
TIGR NTL03PA03297
UniParc UPI00000C599B
UniProtKB Acc Q9HYU6
UniProtKB ID Q9HYU6_PSEAE
UniRef100 UniRef100_U9IQK5
UniRef50 UniRef50_S6AM29
UniRef90 UniRef90_V6AC29

Product

Feature Type CDS
Coding Frame 1
Product Name
probable ATP-dependent helicase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 11.14
Kyte-Doolittle Hydrophobicity Value -0.404
Molecular Weight (kDa) 149.9
Isoelectric Point (pI) 8.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 314 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002319 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3297

Human Homologs

Ensembl 110, assembly GRCh38.p14
DEAH-box helicase 16 [Source:HGNC Symbol;Acc:HGNC:2739]
E-value: 1.5e-116
Percent Identity: 38.8

References

Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae.
Moriya H, Kasai H, Isono K
Nucleic Acids Res 1995 Feb 25;23(4):595-8
PubMed ID: 7899078
PA3297 Counteracts Antimicrobial Effects of Azithromycin in Pseudomonas aeruginosa.
Tan H, Zhang L, Weng Y, Chen R, Zhu F, Jin Y, Cheng Z, Jin S, Wu W
Front Microbiol 2016;7:317
PubMed ID: 27014238