Pseudomonas aeruginosa PAO1, PA3297

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3297
Name
Synonym: hrpA
Replicon chromosome
Genomic location 3691024 - 3695004 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251987.1
GI 15598493
Affymetrix PA3297_at
Entrez 882460
GenBank AAG06685.1
INSDC AAG06685.1
NCBI Locus Tag PA3297
protein_id(GenBank) gb|AAG06685.1|AE004752_1|gnl|PseudoCAP|PA3297
TIGR NTL03PA03297
UniParc UPI00000C599B
UniProtKB Acc Q9HYU6
UniProtKB ID Q9HYU6_PSEAE
UniRef100 UniRef100_U9IQK5
UniRef50 UniRef50_S6AM29
UniRef90 UniRef90_V6AC29

Product

Feature Type CDS
Coding Frame 1
Product Name
probable ATP-dependent helicase
Product Name Confidence: Class 3
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 11.14
Kyte-Doolittle Hydrophobicity Value -0.404
Molecular Weight (kDa) 149.9
Isoelectric Point (pI) 8.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 314 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002319 (572 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3297

Human Homologs

Ensembl 86, assembly GRCh38.p7
DEAH-box helicase 15 [Source:HGNC Symbol;Acc:HGNC:2738]
E-value: 2.0e-119
Percent Identity: 36.6

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 21043
Genomic Position 3693269
Position in ORF 2246(3981)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 1358
Genomic Position 3693494
Position in ORF 2471(3981)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_21400
Mutant ID PA14NR: 35180
Genomic Position 1855417
Position in ORF 3198(3981)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_21400
Mutant ID PA14NR: 37804
Genomic Position 1853459
Position in ORF 1240(3981)
Reference 16477005

References

Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae.
Moriya H, Kasai H, Isono K.
Nucleic Acids Res. 1995 Feb 25;23(4):595-8.
PubMed ID: 7899078
PA3297 Counteracts Antimicrobial Effects of Azithromycin in Pseudomonas aeruginosa.
Tan H(1), Zhang L(1), Weng Y(1), Chen R(1), Zhu F(1), Jin Y(1), Cheng Z(1), Jin S(2), Wu W(1).
Front Microbiol. 2016 Mar 16;7:317.
PubMed ID: 27014238