Pseudomonas aeruginosa PAO1, PA3343 (hsbD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3343
Name
hsbD
Replicon chromosome
Genomic location 3753763 - 3754932 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_252033.1
GI 15598539
Affymetrix PA3343_at
Entrez 882508
GenBank AAG06731.1
INSDC AAG06731.1
NCBI Locus Tag PA3343
protein_id(GenBank) gb|AAG06731.1|AE004756_9|gnl|PseudoCAP|PA3343
TIGR NTL03PA03343
UniParc UPI00000C59C5
UniProtKB Acc Q9HYQ2
UniProtKB ID Q9HYQ2_PSEAE
UniRef100 UniRef100_Q9HYQ2
UniRef50 UniRef50_Q9HYQ2
UniRef90 UniRef90_Q9HYQ2

Product

Feature Type CDS
Coding Frame 1
Product Name
diguanylate cyclase HsbD
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) 4.40
Kyte-Doolittle Hydrophobicity Value 0.215
Molecular Weight (kDa) 43.6
Isoelectric Point (pI) 8.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 452 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002285 (678 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3343
Search term: hsbD

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 3783
Genomic Position 3753948
Position in ORF 186(1170)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 50949
Genomic Position 3753940
Position in ORF 178(1170)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Kulasakara H, Lee V, Brencic A, Liberati N, Urbach J, Miyata S, Lee DG, Neely AN, Hyodo M, Hayakawa Y, Ausubel FM, Lory S
Proc. Natl. Acad. Sci. U.S.A. 2006 Feb;103(8):2839-44
PubMed ID: 16477007
The Diguanylate Cyclase HsbD Intersects with the HptB Regulatory Cascade to Control Pseudomonas aeruginosa Biofilm and Motility.
Valentini M, Laventie BJ, Moscoso J, Jenal U, Filloux A
PLoS Genet. 2016 Oct;12(10):e1006354
PubMed ID: 27792789