Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3345
|
Name |
hptB
|
Replicon | chromosome |
Genomic location | 3757243 - 3757593 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Using in vitro phosphorylation and bacterial two hybrid analysis, Lin et al. and Hsu et al. subsequently showed that HptB can be phosphorylated by four hybrid sensors, PA1611, PA1976, PA2824 and RetS, and that HptB can transfer a phosphate onto the adjacent response regulator PA3346. |
Comment | . It was further shown that the HptB signalling pathway intersects the GacA signalling pathway that inversely controls the expression the pel genes involved in biofilm formation and the type III secretion system. |
Comment | using genetic, bacterial two hybrid and biochemical analyses, Bordi et al. showed that HptB actually has a negative effect on the activity of the PA3346 response regulator, the function of which is to activate the anti-?-factor PA3347. |
RefSeq | NP_252035.1 |
GI | 15598541 |
Affymetrix | PA3345_at |
DNASU | PaCD00007464 |
Entrez | 882510 |
GenBank | AAG06733.1 |
INSDC | AAG06733.1 |
NCBI Locus Tag | PA3345 |
protein_id(GenBank) | gb|AAG06733.1|AE004757_2|gnl|PseudoCAP|PA3345 |
TIGR | NTL03PA03345 |
UniParc | UPI00000C59C7 |
UniProtKB Acc | Q9HYQ0 |
UniProtKB ID | Q9HYQ0_PSEAE |
UniRef100 | UniRef100_Q9HYQ0 |
UniRef50 | UniRef50_Q9HYQ0 |
UniRef90 | UniRef90_Q9HYQ0 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Histidine phosphotransfer protein HptB
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -3.08 |
Kyte-Doolittle Hydrophobicity Value | -0.396 |
Molecular Weight (kDa) | 13.2 |
Isoelectric Point (pI) | 5.64 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7C1I | TRANSFERASE | 05/04/20 | Crystal structure of histidine-containing phosphotransfer protein B (HptB) from Pseudomonas aeruginosa PAO1 | Pseudomonas aeruginosa | 1.58 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 83 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002283 (519 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3345
Search term: hptB
Search term: Histidine phosphotransfer protein HptB
|
Two-component systems in Pseudomonas aeruginosa: why so many?
Rodrigue A, Quentin Y, Lazdunski A, Méjean V, Foglino M
Trends Microbiol 2000 Nov;8(11):498-504
PubMed ID: 11121759
|
Identification of an HptB-mediated multi-step phosphorelay in Pseudomonas aeruginosa PAO1.
Lin CT, Huang YJ, Chu PH, Hsu JL, Huang CH, Peng HL
Res Microbiol 2006 Mar;157(2):169-75
PubMed ID: 16182517
|
Characterization of the histidine-containing phosphotransfer protein B-mediated multistep phosphorelay system in Pseudomonas aeruginosa PAO1.
Hsu JL, Chen HC, Peng HL, Chang HY
J Biol Chem 2008 Apr 11;283(15):9933-44
PubMed ID: 18256026
|
Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis.
Bordi C, Lamy MC, Ventre I, Termine E, Hachani A, Fillet S, Roche B, Bleves S, Méjean V, Lazdunski A, Filloux A
Mol Microbiol 2010 Jun;76(6):1427-43
PubMed ID: 20398205
|
Histidine-containing phosphotransfer protein-B (HptB) regulates swarming motility through partner-switching system in Pseudomonas aeruginosa PAO1 strain.
Bhuwan M, Lee HJ, Peng HL, Chang HY
J Biol Chem 2012 Jan 13;287(3):1903-14
PubMed ID: 22128156
|