Pseudomonas aeruginosa PAO1, PA3345 (hptB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3345
Name
hptB
Replicon chromosome
Genomic location 3757243 - 3757593 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentUsing in vitro phosphorylation and bacterial two hybrid analysis, Lin et al. and Hsu et al. subsequently showed that HptB can be phosphorylated by four hybrid sensors, PA1611, PA1976, PA2824 and RetS, and that HptB can transfer a phosphate onto the adjacent response regulator PA3346.
Comment. It was further shown that the HptB signalling pathway intersects the GacA signalling pathway that inversely controls the expression the pel genes involved in biofilm formation and the type III secretion system.
Commentusing genetic, bacterial two hybrid and biochemical analyses, Bordi et al. showed that HptB actually has a negative effect on the activity of the PA3346 response regulator, the function of which is to activate the anti-?-factor PA3347.

Cross-References

RefSeq NP_252035.1
GI 15598541
Affymetrix PA3345_at
DNASU PaCD00007464
Entrez 882510
GenBank AAG06733.1
INSDC AAG06733.1
NCBI Locus Tag PA3345
protein_id(GenBank) gb|AAG06733.1|AE004757_2|gnl|PseudoCAP|PA3345
TIGR NTL03PA03345
UniParc UPI00000C59C7
UniProtKB Acc Q9HYQ0
UniProtKB ID Q9HYQ0_PSEAE
UniRef100 UniRef100_Q9HYQ0
UniRef50 UniRef50_Q9HYQ0
UniRef90 UniRef90_Q9HYQ0

Product

Feature Type CDS
Coding Frame 1
Product Name
Histidine phosphotransfer protein HptB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.08
Kyte-Doolittle Hydrophobicity Value -0.396
Molecular Weight (kDa) 13.2
Isoelectric Point (pI) 5.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7C1I TRANSFERASE 05/04/20 Crystal structure of histidine-containing phosphotransfer protein B (HptB) from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa 1.58 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 83 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002283 (519 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3345
Search term: hptB

Human Homologs

References

Two-component systems in Pseudomonas aeruginosa: why so many?
Rodrigue A, Quentin Y, Lazdunski A, Méjean V, Foglino M
Trends Microbiol 2000 Nov;8(11):498-504
PubMed ID: 11121759
Identification of an HptB-mediated multi-step phosphorelay in Pseudomonas aeruginosa PAO1.
Lin CT, Huang YJ, Chu PH, Hsu JL, Huang CH, Peng HL
Res Microbiol 2006 Mar;157(2):169-75
PubMed ID: 16182517
Characterization of the histidine-containing phosphotransfer protein B-mediated multistep phosphorelay system in Pseudomonas aeruginosa PAO1.
Hsu JL, Chen HC, Peng HL, Chang HY
J Biol Chem 2008 Apr 11;283(15):9933-44
PubMed ID: 18256026
Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis.
Bordi C, Lamy MC, Ventre I, Termine E, Hachani A, Fillet S, Roche B, Bleves S, Méjean V, Lazdunski A, Filloux A
Mol Microbiol 2010 Jun;76(6):1427-43
PubMed ID: 20398205
Histidine-containing phosphotransfer protein-B (HptB) regulates swarming motility through partner-switching system in Pseudomonas aeruginosa PAO1 strain.
Bhuwan M, Lee HJ, Peng HL, Chang HY
J Biol Chem 2012 Jan 13;287(3):1903-14
PubMed ID: 22128156