Pseudomonas aeruginosa PAO1, PA3346 (hsbR)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 3757659 - 3759374 (- strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_252036.1
GI 15598542
Affymetrix PA3346_at
Entrez 882513
GenBank AAG06734.1
INSDC AAG06734.1
NCBI Locus Tag PA3346
protein_id(GenBank) gb|AAG06734.1|AE004757_3|gnl|PseudoCAP|PA3346
UniParc UPI00000C59C8
UniProtKB Acc Q9HYP9
UniRef100 UniRef100_W0YSZ1
UniRef50 UniRef50_G8Q0M8
UniRef90 UniRef90_W0YSZ1


Feature Type CDS
Coding Frame 1
Product Name
HptB-dependent secretion and biofilm regulator HsbR
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -11.81
Kyte-Doolittle Hydrophobicity Value -0.012
Molecular Weight (kDa) 63.1
Isoelectric Point (pI) 5.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 624 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002282 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3346
Search term: hsbR

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 14739
Genomic Position 3758811
Position in ORF 1153(1716)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 11781
Genomic Position 3759214
Position in ORF 1556(1716)
Reference 14617778

Transposon Insertions in Orthologs


A putative two-component regulatory system involved in secondary metabolism in Streptomyces spp.
Ishizuka H, Horinouchi S, Kieser HM, Hopwood DA, Beppu T
J. Bacteriol. 1992 Dec;174(23):7585-94
PubMed ID: 1339426
Histidine-containing phosphotransfer protein-B (HptB) regulates swarming motility through partner-switching system in Pseudomonas aeruginosa PAO1 strain.
Bhuwan M, Lee HJ, Peng HL, Chang HY
J. Biol. Chem. 2012 Jan;287(3):1903-14
PubMed ID: 22128156
Identification of an HptB-mediated multi-step phosphorelay in Pseudomonas aeruginosa PAO1.
Lin CT, Huang YJ, Chu PH, Hsu JL, Huang CH, Peng HL
Res. Microbiol. 2006 Mar;157(2):169-75
PubMed ID: 16182517
Characterization of the histidine-containing phosphotransfer protein B-mediated multistep phosphorelay system in Pseudomonas aeruginosa PAO1.
Hsu JL, Chen HC, Peng HL, Chang HY
J. Biol. Chem. 2008 Apr;283(15):9933-44
PubMed ID: 18256026
A bacterial two-hybrid genome fragment library for deciphering regulatory networks of the opportunistic pathogen Pseudomonas aeruginosa.
Houot L, Fanni A, de Bentzmann S, Bordi C
Microbiology (Reading, Engl.) 2012 Aug;158(Pt 8):1964-71
PubMed ID: 22628483