Pseudomonas aeruginosa PAO1, PA3347 (hsbA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 3759376 - 3759681 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_252037.1
GI 15598543
Affymetrix PA3347_at
DNASU PaCD00007465
Entrez 882514
GenBank AAG06735.1
INSDC AAG06735.1
NCBI Locus Tag PA3347
protein_id(GenBank) gb|AAG06735.1|AE004757_4|gnl|PseudoCAP|PA3347
UniParc UPI00000C59C9
UniProtKB Acc Q9HYP8
UniRef100 UniRef100_Q02QI1
UniRef50 UniRef50_Q02QI1
UniRef90 UniRef90_Q02QI1


Feature Type CDS
Coding Frame 1
Product Name
HptB-dependent secretion and biofilm anti anti-sigma factor HsbA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.57
Kyte-Doolittle Hydrophobicity Value -0.063
Molecular Weight (kDa) 11.2
Isoelectric Point (pI) 5.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 69 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002281 (543 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3347
Search term: hsbA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 37778
Genomic Position 3759529
Position in ORF 154(306)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 49584
Genomic Position 3759428
Position in ORF 53(306)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_20770
Mutant ID PA14NR: 39911
Genomic Position 1787683
Position in ORF 130(306)
Reference 16477005


Histidine-containing phosphotransfer protein-B (HptB) regulates swarming motility through partner-switching system in Pseudomonas aeruginosa PAO1 strain.
Bhuwan M, Lee HJ, Peng HL, Chang HY
J. Biol. Chem. 2012 Jan;287(3):1903-14
PubMed ID: 22128156
A bacterial two-hybrid genome fragment library for deciphering regulatory networks of the opportunistic pathogen Pseudomonas aeruginosa.
Houot L, Fanni A, de Bentzmann S, Bordi C
Microbiology (Reading, Engl.) 2012 Aug;158(Pt 8):1964-71
PubMed ID: 22628483
The Diguanylate Cyclase HsbD Intersects with the HptB Regulatory Cascade to Control Pseudomonas aeruginosa Biofilm and Motility.
Valentini M, Laventie BJ, Moscoso J, Jenal U, Filloux A
PLoS Genet. 2016 Oct;12(10):e1006354
PubMed ID: 27792789