Pseudomonas aeruginosa PAO1, PA3357 (dsdA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3357
Name
dsdA
Replicon chromosome
Genomic location 3768184 - 3769530 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252047.1
GI 15598553
Affymetrix PA3357_dsdA_at
Entrez 882524
GenBank AAG06745.1
INSDC AAG06745.1
NCBI Locus Tag PA3357
protein_id(GenBank) gb|AAG06745.1|AE004758_2|gnl|PseudoCAP|PA3357
TIGR NTL03PA03357
UniParc UPI000013572C
UniProtKB Acc Q9HYN9
UniProtKB ID SDHD_PSEAE
UniRef100 UniRef100_Q9HYN9
UniRef50 UniRef50_A0RCF8
UniRef90 UniRef90_Q9HYN9

Product

Feature Type CDS
Coding Frame 1
Product Name
D-serine dehydratase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.50
Kyte-Doolittle Hydrophobicity Value -0.034
Molecular Weight (kDa) 48.2
Isoelectric Point (pI) 5.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 103 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002271 (377 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3357
Search term: dsdA

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 19747
Genomic Position 3768247
Position in ORF 64(1347)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 17266
Genomic Position 3768752
Position in ORF 569(1347)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_20650
Mutant ID PA14NR: 44591
Genomic Position 1778610
Position in ORF 906(1347)
Reference 16477005

References

DNA sequences of the D-serine deaminase control region and N-terminal portion of the structural gene.
McFall E, Runkel L.
J Bacteriol. 1983 Jun;154(3):1508-12.
PubMed ID: 6343359
D-serine dehydratase from Escherichia coli. DNA sequence and identification of catalytically inactive glycine to aspartic acid variants.
Marceau M, McFall E, Lewis SD, Shafer JA.
J Biol Chem. 1988 Nov 15;263(32):16926-33.
PubMed ID: 3053699
The Cryptic dsdA Gene Encodes a Functional D-Serine Dehydratase in Pseudomonas aeruginosa PAO1.
Li G(1,)(2), Lu CD(3,)(4).
Curr Microbiol. 2016 Jun;72(6):788-94.
PubMed ID: 26957519