Pseudomonas aeruginosa PAO1, PA3365

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3365
Name
Synonym: amiB
Replicon chromosome
Genomic location 3775761 - 3776876 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252055.1
GI 15598561
Affymetrix PA3365_at
Entrez 877799
GenBank AAG06753.1
INSDC AAG06753.1
NCBI Locus Tag PA3365
protein_id(GenBank) gb|AAG06753.1|AE004758_10|gnl|PseudoCAP|PA3365
TIGR NTL03PA03365
UniParc UPI000012597C
UniProtKB Acc Q51416
UniProtKB ID AMIB_PSEAE
UniRef100 UniRef100_Q51416
UniRef50 UniRef50_Q51416
UniRef90 UniRef90_Q51416

Product

Feature Type CDS
Coding Frame 1
Product Name
probable chaperone
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 6.24
Kyte-Doolittle Hydrophobicity Value -0.385
Molecular Weight (kDa) 42.1
Isoelectric Point (pI) 8.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 597 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002263 (346 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3365

Human Homologs

Ensembl 86, assembly GRCh38.p7
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 1.5e-24
Percent Identity: 29.8

References

Identification of two new genes in the Pseudomonas aeruginosa amidase operon, encoding an ATPase (AmiB) and a putative integral membrane protein (AmiS).
Wilson SA, Williams RJ, Pearl LH, Drew RE
J. Biol. Chem. 1995 Aug;270(32):18818-24
PubMed ID: 7642533