Pseudomonas aeruginosa PAO1, PA3408 (hasR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3408
Name
hasR
Replicon chromosome
Genomic location 3814661 - 3817336 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252098.1
GI 15598604
Affymetrix PA3408_hasR_at
Entrez 879855
GenBank AAG06796.1
INSDC AAG06796.1
NCBI Locus Tag PA3408
protein_id(GenBank) gb|AAG06796.1|AE004762_2|gnl|PseudoCAP|PA3408
TIGR NTL03PA03408
UniParc UPI00000C59F6
UniProtKB Acc Q9HYJ7
UniProtKB ID Q9HYJ7_PSEAE
UniRef100 UniRef100_Q9HYJ7
UniRef50 UniRef50_Q4K5N7
UniRef90 UniRef90_Q9HYJ7

Product

Feature Type CDS
Coding Frame 1
Product Name
Heme uptake outer membrane receptor HasR precursor
Product Name Confidence: Class 1
Synonyms Haem uptake outer membrane receptor HasR precursor
Evidence for Translation
Charge (pH 7) -3.91
Kyte-Doolittle Hydrophobicity Value -0.386
Molecular Weight (kDa) 97.9
Isoelectric Point (pI) 6.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 190 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002222 (348 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3408
Search term: hasR

Human Homologs

References

Genetics and regulation of two distinct haem-uptake systems, phu and has, in Pseudomonas aeruginosa.
Ochsner UA, Johnson Z, Vasil ML
Microbiology (Reading) 2000 Jan;146 ( Pt 1):185-198
PubMed ID: 10658665
A new type of hemophore-dependent heme acquisition system of Serratia marcescens reconstituted in Escherichia coli.
Ghigo JM, Létoffé S, Wandersman C
J Bacteriol 1997 Jun;179(11):3572-9
PubMed ID: 9171402
Differential contributions of the outer membrane receptors PhuR and HasR to heme acquisition in Pseudomonas aeruginosa.
Smith AD, Wilks A
J Biol Chem 2015 Mar 20;290(12):7756-66
PubMed ID: 25616666