Pseudomonas aeruginosa PAO1, PA3450 (lsfA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3450
Name
lsfA
Replicon chromosome
Genomic location 3855493 - 3856131 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252140.1
GI 15598646
Affymetrix PA3450_at
Entrez 877870
GenBank AAG06838.1
INSDC AAG06838.1
NCBI Locus Tag PA3450
protein_id(GenBank) gb|AAG06838.1|AE004765_11|gnl|PseudoCAP|PA3450
TIGR NTL03PA03450
UniParc UPI00000C5A1A
UniProtKB Acc Q9HYF6
UniProtKB ID Q9HYF6_PSEAE
UniRef100 UniRef100_S0J3K2
UniRef50 UniRef50_Q54SE2
UniRef90 UniRef90_A0A024HLA3

Product

Feature Type CDS
Coding Frame 1
Product Name
1-Cys peroxiredoxin LsfA
Product Name Confidence: Class 1
Synonyms probable antioxidant protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.83
Kyte-Doolittle Hydrophobicity Value -0.410
Molecular Weight (kDa) 24.2
Isoelectric Point (pI) 5.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7KUU OXIDOREDUCTASE 11/25/20 LsfA from P. aeruginosa, a 1-Cys Prx in Sulfonic acid form Pseudomonas aeruginosa (strain UCBPP-PA14) 2 X-RAY DIFFRACTION 99.0
6P0W OXIDOREDUCTASE 05/17/19 LsfA from P. aeruginosa, a 1-Cys Prx in reduced form Pseudomonas aeruginosa (strain UCBPP-PA14) 2.4 X-RAY DIFFRACTION 99.1

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 593 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002190 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3450
Search term: lsfA

Human Homologs

Ensembl 110, assembly GRCh38.p14
peroxiredoxin 6 [Source:HGNC Symbol;Acc:HGNC:16753]
E-value: 1.9e-61
Percent Identity: 53.4

References

No references are associated with this feature.