Pseudomonas aeruginosa PAO1, PA3479 (rhlA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3479
Name
rhlA
Replicon chromosome
Genomic location 3892121 - 3893008 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentRhlA condenses two beta-hyroxydecanoly-ACPs to form the beta-hydroxydecanoly-beta-hydroxyldecanoate moiety of rhamnolipids

Cross-References

RefSeq NP_252169.1
GI 15598675
Affymetrix PA3479_rhlA_at
DNASU PaCD00007528
Entrez 878955
GenBank AAG06867.1
INSDC AAG06867.1
NCBI Locus Tag PA3479
protein_id(GenBank) gb|AAG06867.1|AE004768_12|gnl|PseudoCAP|PA3479
TIGR NTL03PA03479
UniParc UPI0000133874
UniProtKB Acc Q51559
UniProtKB ID RHLA_PSEAE
UniRef100 UniRef100_Q51559
UniRef50 UniRef50_Q51559
UniRef90 UniRef90_Q51559

Product

Feature Type CDS
Coding Frame 1
Product Name
rhamnosyltransferase chain A
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 2.45
Kyte-Doolittle Hydrophobicity Value -0.180
Molecular Weight (kDa) 33.0
Isoelectric Point (pI) 7.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Rhamnolipid biosynthesis
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Homo sapiens
Notes In vitro biofilms of P. aeruginosa PAO1 rhlA mutants yield fewer necrotic PMNs than wild-type PAO1.
Reference 19594494
Source 2
Database VFDB : VF0089
Category Rhamnolipid biosynthesis
Evidence
Host Organism
Notes
Reference
Source 3
Database PseudoCAP
Category Rhamnolipid biosynthesis
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model pulmonary Infection model
Infection model foreign-body Infection model
Reference 19594494

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 22 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002163 (333 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3479
Search term: rhlA

Human Homologs

References

RhlA converts beta-hydroxyacyl-acyl carrier protein intermediates in fatty acid synthesis to the beta-hydroxydecanoyl-beta-hydroxydecanoate component of rhamnolipids in Pseudomonas aeruginosa.
Zhu K, Rock CO
J Bacteriol 2008 May;190(9):3147-54
PubMed ID: 18326581