Pseudomonas aeruginosa PAO1, PA3484 (tse3)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3484
Name	tse3
Replicon	chromosome
Genomic location	3898278 - 3899504 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_252174.1
GI	15598680
Affymetrix	PA3484_at
Entrez	880027
GenBank	AAG06872.1
INSDC	AAG06872.1
NCBI Locus Tag	PA3484
protein_id(GenBank)	gb|AAG06872.1|AE004769_5|gnl|PseudoCAP|PA3484
TIGR	NTL03PA03484
UniParc	UPI00000C5A32
UniProtKB Acc	Q9HYC5
UniProtKB ID	Q9HYC5_PSEAE
UniRef100	UniRef100_Q9HYC5
UniRef50	UniRef50_Q9HYC5
UniRef90	UniRef90_Q9HYC5

Product

Feature Type	CDS
Coding Frame	1
Product Name	Tse3 Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	2.97
Kyte-Doolittle Hydrophobicity Value	-0.203
Molecular Weight (kDa)	44.4
Isoelectric Point (pI)	9.07

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543 Host Associated Class 1 Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3WA5	HYDROLASE	04/26/13	Crystal Structure of type VI peptidoglycan muramidase effector Tse3 in complex with its cognate immunity protein Tsi3	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	100.0
4N80	HYDROLASE/HYDROLASE INHIBITOR	10/16/13	Crystal structure of Tse3-Tsi3 complex	Pseudomonas aeruginosa	2.4	X-RAY DIFFRACTION	100.0
4N7S	HYDROLASE/HYDROLASE INHIBITOR	10/16/13	Crystal structure of Tse3-Tsi3 complex with Zinc ion	Pseudomonas aeruginosa	2.101	X-RAY DIFFRACTION	100.0
4N88	HYDROLASE/HYDROLASE INHIBITOR	10/17/13	Crystal structure of Tse3-Tsi3 complex with calcium ion	Pseudomonas aeruginosa	2.8	X-RAY DIFFRACTION	100.0
4M5E	HYDROLASE	08/08/13	Tse3 structure	Pseudomonas aeruginosa	1.49	X-RAY DIFFRACTION	100.0
4M5F	HYDROLASE INHIBITOR	08/08/13	complex structure of Tse3-Tsi3	Pseudomonas aeruginosa	2.5	X-RAY DIFFRACTION	100.0
4LUQ	PROTEIN BINDING/TOXIN INHIBITOR	07/25/13	Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3	Pseudomonas aeruginosa	1.77	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 2 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002158 (298 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3484 Search term: tse3 Search term: Tse3

Human Homologs

References

Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli. Striebel HM, Seeber S, Jarsch M, Kessler C Gene 1996 Jun 12;172(1):41-6 PubMed ID: 8654988

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD Cell Host Microbe 2010 Jan 21;7(1):25-37 PubMed ID: 20114026

Type VI secretion delivers bacteriolytic effectors to target cells. Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD Nature 2011 Jul 20;475(7356):343-7 PubMed ID: 21776080