Pseudomonas aeruginosa PAO1, PA3487 (tle5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3487
Name
tle5
Replicon chromosome
Genomic location 3902663 - 3905962 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252177.1
GI 15598683
Affymetrix PA3487_at
Entrez 880040
GenBank AAG06875.1
INSDC AAG06875.1
NCBI Locus Tag PA3487
protein_id(GenBank) gb|AAG06875.1|AE004769_8|gnl|PseudoCAP|PA3487
TIGR NTL03PA03487
UniParc UPI00000C5A35
UniProtKB Acc Q9HYC2
UniProtKB ID Q9HYC2_PSEAE
UniRef100 UniRef100_Q9HYC2
UniRef50 UniRef50_Q9HYC2
UniRef90 UniRef90_Q9HYC2

Product

Feature Type CDS
Coding Frame 1
Product Name
Tle5
Product Name Confidence: Class 1
Synonyms PldA
Evidence for Translation
Charge (pH 7) -3.85
Kyte-Doolittle Hydrophobicity Value -0.316
Molecular Weight (kDa) 122.3
Isoelectric Point (pI) 6.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Host Associated Class 1
Periplasmic Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7V55 HYDROLASE 08/16/21 Crystal structure of phospholipase D from Pseudomonas aeruginosa PAO1 using in situ proteolysis Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3 X-RAY DIFFRACTION 100.0
7WDK IMMUNE SYSTEM 12/21/21 The structure of PldA-PA3488 complex Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.05 ELECTRON MICROSCOPY 100.0
7V53 HYDROLASE 08/16/21 Crystal structure of full-length phospholipase D from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.1 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB
Category Phospholipase D
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 11136451

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 26 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002156 (79 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3487
Search term: tle5
Search term: Tle5

Human Homologs

Ensembl 110, assembly GRCh38.p14
phospholipase D1 [Source:HGNC Symbol;Acc:HGNC:9067]
E-value: 7.9e-26
Percent Identity: 30.7
Ensembl 110, assembly GRCh38.p14
phospholipase D1 [Source:HGNC Symbol;Acc:HGNC:9067]
E-value: 7.9e-26
Percent Identity: 30.7

References

Genetic and biochemical analyses of a eukaryotic-like phospholipase D of Pseudomonas aeruginosa suggest horizontal acquisition and a role for persistence in a chronic pulmonary infection model.
Wilderman PJ, Vasil AI, Johnson Z, Vasil ML
Mol Microbiol 2001 Jan;39(2):291-303
PubMed ID: 11136451
Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
Nature 2013 Apr 25;496(7446):508-12
PubMed ID: 23552891
A Pseudomonas aeruginosa type VI secretion phospholipase D effector targets both prokaryotic and eukaryotic cells.
Jiang F, Waterfield NR, Yang J, Yang G, Jin Q
Cell Host Microbe 2014 May 14;15(5):600-10
PubMed ID: 24832454
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A
Proc Natl Acad Sci U S A 2017 Jul 18;114(29):7707-7712
PubMed ID: 28673999
Biochemical characterization of a Pseudomonas aeruginosa phospholipase D.
Spencer C, Brown HA
Biochemistry 2015 Feb 10;54(5):1208-18
PubMed ID: 25565226