Pseudomonas aeruginosa PAO1, PA3521 (opmE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3521
Name
opmE
Replicon chromosome
Genomic location 3938020 - 3939495 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252211.1
GI 15598717
Affymetrix PA3521_at
Entrez 878905
GenBank AAG06909.1
INSDC AAG06909.1
NCBI Locus Tag PA3521
protein_id(GenBank) gb|AAG06909.1|AE004772_7|gnl|PseudoCAP|PA3521
TIGR NTL03PA03521
UniParc UPI0000001FDA
UniProtKB Acc Q9HY88
UniProtKB ID Q9HY88_PSEAE
UniRef100 UniRef100_G3XCL7
UniRef50 UniRef50_I2BMT2
UniRef90 UniRef90_G3XCL7

Product

Feature Type CDS
Coding Frame 1
Product Name
OpmE
Product Name Confidence: Class 3
Synonyms probable outer membrane protein precursor
Evidence for Translation
Charge (pH 7) -7.33
Kyte-Doolittle Hydrophobicity Value -0.163
Molecular Weight (kDa) 52.8
Isoelectric Point (pI) 5.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
opmE opmE is an outer membrane factor protein that is part of the multidrug efflux pump MexPQ-OpmE. [PMID:16301811] ARO:3003700 protein homolog model Perfect 927.2 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 285 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002143 (311 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3521
Search term: opmE
Search term: OpmE

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
Köhler T, Michéa-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechère JC
Mol Microbiol 1997 Jan;23(2):345-54
PubMed ID: 9044268
Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa.
Mima T, Sekiya H, Mizushima T, Kuroda T, Tsuchiya T
Microbiol Immunol 2005;49(11):999-1002
PubMed ID: 16301811