Pseudomonas aeruginosa PAO1, PA3521 (opmE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3521
Name
opmE
Replicon chromosome
Genomic location 3938020 - 3939495 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252211.1
GI 15598717
Affymetrix PA3521_at
Entrez 878905
GenBank AAG06909.1
INSDC AAG06909.1
NCBI Locus Tag PA3521
protein_id(GenBank) gb|AAG06909.1|AE004772_7|gnl|PseudoCAP|PA3521
TIGR NTL03PA03521
UniParc UPI0000001FDA
UniProtKB Acc Q9HY88
UniProtKB ID Q9HY88_PSEAE
UniRef100 UniRef100_G3XCL7
UniRef50 UniRef50_I2BMT2
UniRef90 UniRef90_G3XCL7

Product

Feature Type CDS
Coding Frame 1
Product Name
OpmE
Product Name Confidence: Class 3
Evidence for Translation
Charge (pH 7) -7.33
Kyte-Doolittle Hydrophobicity Value -0.163
Molecular Weight (kDa) 52.8
Isoelectric Point (pI) 5.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 285 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002143 (320 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3521
Search term: opmE
Search term: OpmE

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 42288
Genomic Position 3938297
Position in ORF 278(1476)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 42105
Genomic Position 3938364
Position in ORF 345(1476)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_18790
Mutant ID PA14NR: 32615
Genomic Position 1619019
Position in ORF 379(1476)
Reference 16477005

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PubMed ID: 15687295
Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
Kohler T, Michea-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechère JC.
Mol Microbiol. 1997 Jan;23(2):345-54.
PubMed ID: 9044268
Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa.
Mima T(1), Sekiya H, Mizushima T, Kuroda T, Tsuchiya T.
Microbiol Immunol. 2005;49(11):999-1002.
PubMed ID: 16301811