Pseudomonas aeruginosa PAO1, PA3530 (bfd)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3530
Name
bfd
Synonym: bfd
Replicon chromosome
Genomic location 3949853 - 3950074 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252220.1
GI 15598726
Affymetrix PA3530_at
DNASU PaCD00007552
Entrez 879943
GenBank AAG06918.1
INSDC AAG06918.1
NCBI Locus Tag PA3530
protein_id(GenBank) gb|AAG06918.1|AE004773_7|gnl|PseudoCAP|PA3530
TIGR NTL03PA03530
UniParc UPI00000C5A56
UniProtKB Acc Q9HY80
UniProtKB ID Q9HY80_PSEAE
UniRef100 UniRef100_W0YVW9
UniRef50 UniRef50_F6AEI0
UniRef90 UniRef90_W0YVW9

Product

Feature Type CDS
Coding Frame 1
Product Name
bacterioferritin-associated ferredoxin Bfd
Product Name Confidence: Class 1
Synonyms bacterioferritin-associated ferredoxin
Bfd
Evidence for Translation
Charge (pH 7) -1.23
Kyte-Doolittle Hydrophobicity Value 0.074
Molecular Weight (kDa) 7.8
Isoelectric Point (pI) 4.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6E6S ELECTRON TRANSPORT 07/25/18 1.45 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) R26E/K46Y mutant from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.45 X-RAY DIFFRACTION 94.6
4E6K METAL BINDING PROTEIN/ELECTRON TRANSPORT 03/15/12 2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd) Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 98.6
6E6R ELECTRON TRANSPORT 07/25/18 1.50 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) R26E mutant from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 96.4
6E6Q ELECTRON TRANSPORT 07/25/18 1.20 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.2 X-RAY DIFFRACTION 98.2

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 93 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002135 (514 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3530
Search term: bfd

Human Homologs

References

A [2Fe-2S] protein encoded by an open reading frame upstream of the Escherichia coli bacterioferritin gene.
Garg RP, Vargo CJ, Cui X, Kurtz DM Jr
Biochemistry 1996 May 21;35(20):6297-301
PubMed ID: 8639572
Spectroscopic and voltammetric characterisation of the bacterioferritin-associated ferredoxin of Escherichia coli.
Quail MA, Jordan P, Grogan JM, Butt JN, Lutz M, Thomson AJ, Andrews SC, Guest JR
Biochem Biophys Res Commun 1996 Dec 13;229(2):635-42
PubMed ID: 8954950
Biochemical and structural characterization of Pseudomonas aeruginosa Bfd and FPR: ferredoxin NADP+ reductase and not ferredoxin is the redox partner of heme oxygenase under iron-starvation conditions.
Wang A, Zeng Y, Han H, Weeratunga S, Morgan BN, Moënne-Loccoz P, Schönbrunn E, Rivera M
Biochemistry 2007 Oct 30;46(43):12198-211
PubMed ID: 17915950
The structure of the BfrB-Bfd complex reveals protein-protein interactions enabling iron release from bacterioferritin.
Yao H, Wang Y, Lovell S, Kumar R, Ruvinsky AM, Battaile KP, Vakser IA, Rivera M
J Am Chem Soc 2012 Aug 15;134(32):13470-81
PubMed ID: 22812654
Binding of Pseudomonas aeruginosa apobacterioferritin-associated ferredoxin to bacterioferritin B promotes heme mediation of electron delivery and mobilization of core mineral iron.
Weeratunga SK, Gee CE, Lovell S, Zeng Y, Woodin CL, Rivera M
Biochemistry 2009 Aug 11;48(31):7420-31
PubMed ID: 19575528