Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3530
|
Name |
bfd
Synonym: bfd |
Replicon | chromosome |
Genomic location | 3949853 - 3950074 (+ strand) |
Transposon Mutants | 4 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_252220.1 |
GI | 15598726 |
Affymetrix | PA3530_at |
DNASU | PaCD00007552 |
Entrez | 879943 |
GenBank | AAG06918.1 |
INSDC | AAG06918.1 |
NCBI Locus Tag | PA3530 |
protein_id(GenBank) | gb|AAG06918.1|AE004773_7|gnl|PseudoCAP|PA3530 |
TIGR | NTL03PA03530 |
UniParc | UPI00000C5A56 |
UniProtKB Acc | Q9HY80 |
UniProtKB ID | Q9HY80_PSEAE |
UniRef100 | UniRef100_W0YVW9 |
UniRef50 | UniRef50_F6AEI0 |
UniRef90 | UniRef90_W0YVW9 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
bacterioferritin-associated ferredoxin Bfd
|
Synonyms |
bacterioferritin-associated ferredoxin Bfd |
Evidence for Translation | |
Charge (pH 7) | -1.23 |
Kyte-Doolittle Hydrophobicity Value | 0.074 |
Molecular Weight (kDa) | 7.8 |
Isoelectric Point (pI) | 4.83 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6E6S | ELECTRON TRANSPORT | 07/25/18 | 1.45 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) R26E/K46Y mutant from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.45 | X-RAY DIFFRACTION | 94.6 |
4E6K | METAL BINDING PROTEIN/ELECTRON TRANSPORT | 03/15/12 | 2.0 A resolution structure of Pseudomonas aeruginosa bacterioferritin (BfrB) in complex with bacterioferritin associated ferredoxin (Bfd) | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 98.6 |
6E6R | ELECTRON TRANSPORT | 07/25/18 | 1.50 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) R26E mutant from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.5 | X-RAY DIFFRACTION | 96.4 |
6E6Q | ELECTRON TRANSPORT | 07/25/18 | 1.20 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.2 | X-RAY DIFFRACTION | 98.2 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 93 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002135 (514 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3530
Search term: bfd
Search term: bacterioferritin-associated ferredoxin Bfd
|
A [2Fe-2S] protein encoded by an open reading frame upstream of the Escherichia coli bacterioferritin gene.
Garg RP, Vargo CJ, Cui X, Kurtz DM Jr
Biochemistry 1996 May 21;35(20):6297-301
PubMed ID: 8639572
|
Spectroscopic and voltammetric characterisation of the bacterioferritin-associated ferredoxin of Escherichia coli.
Quail MA, Jordan P, Grogan JM, Butt JN, Lutz M, Thomson AJ, Andrews SC, Guest JR
Biochem Biophys Res Commun 1996 Dec 13;229(2):635-42
PubMed ID: 8954950
|
Biochemical and structural characterization of Pseudomonas aeruginosa Bfd and FPR: ferredoxin NADP+ reductase and not ferredoxin is the redox partner of heme oxygenase under iron-starvation conditions.
Wang A, Zeng Y, Han H, Weeratunga S, Morgan BN, Moënne-Loccoz P, Schönbrunn E, Rivera M
Biochemistry 2007 Oct 30;46(43):12198-211
PubMed ID: 17915950
|
The structure of the BfrB-Bfd complex reveals protein-protein interactions enabling iron release from bacterioferritin.
Yao H, Wang Y, Lovell S, Kumar R, Ruvinsky AM, Battaile KP, Vakser IA, Rivera M
J Am Chem Soc 2012 Aug 15;134(32):13470-81
PubMed ID: 22812654
|
Binding of Pseudomonas aeruginosa apobacterioferritin-associated ferredoxin to bacterioferritin B promotes heme mediation of electron delivery and mobilization of core mineral iron.
Weeratunga SK, Gee CE, Lovell S, Zeng Y, Woodin CL, Rivera M
Biochemistry 2009 Aug 11;48(31):7420-31
PubMed ID: 19575528
|