Pseudomonas aeruginosa PAO1, PA3531 (bfrB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3531
Name	bfrB
Replicon	chromosome
Genomic location	3950284 - 3950760 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_252221.1
GI	15598727
Affymetrix	PA3531_bfrB_at
DNASU	PaCD00007553
Entrez	879026
GenBank	AAG06919.1
INSDC	AAG06919.1
NCBI Locus Tag	PA3531
protein_id(GenBank)	gb|AAG06919.1|AE004773_8|gnl|PseudoCAP|PA3531
TIGR	NTL03PA03531
UniParc	UPI00000C5A57
UniProtKB Acc	Q9HY79
UniProtKB ID	Q9HY79_PSEAE
UniRef100	UniRef100_Q9HY79
UniRef50	UniRef50_Q59738
UniRef90	UniRef90_Q9HY79

Product

Feature Type	CDS
Coding Frame	1
Product Name	bacterioferritin Product Name Confidence: Class 1
Synonyms	cytochrome b-1 (cytochrome b-557)
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected using LC-MS/MS (18.55 kDa, PMID:21751344) Detected by iTRAQ (PMID:21863142).
Charge (pH 7)	-11.66
Kyte-Doolittle Hydrophobicity Value	-0.520
Molecular Weight (kDa)	18.6
Isoelectric Point (pI)	4.83

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3IS7	ELECTRON TRANSPORT	08/25/09	Structure of mineralized Bfrb from Pseudomonas aeruginosa to 2.1A Resolution	Pseudomonas aeruginosa	2.1	X-RAY DIFFRACTION	100.0
3IS8	ELECTRON TRANSPORT	08/25/09	Structure of mineralized Bfrb soaked with FeSO4 from Pseudomonas aeruginosa to 2.25A Resolution	Pseudomonas aeruginosa	2.25	X-RAY DIFFRACTION	100.0
3ISE	ELECTRON TRANSPORT	08/25/09	Structure of mineralized Bfrb (double soak) from Pseudomonas aeruginosa to 2.8A Resolution	Pseudomonas aeruginosa	2.8	X-RAY DIFFRACTION	100.0
5D8O	OXIDOREDUCTASE	08/17/15	1.90A resolution structure of BfrB (wild-type, C2221 form) from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)	1.9	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 242 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002134 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3531 Search term: bfrB Search term: bacterioferritin

Human Homologs

References

Amino acid sequence of the bacterioferritin (cytochrome b1) of Escherichia coli-K12. Andrews SC, Smith JM, Guest JR, Harrison PM Biochem Biophys Res Commun 1989 Jan 31;158(2):489-96 PubMed ID: 2644932

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB Front Microbiol 2011;2:162 PubMed ID: 21863142

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

Two distinct ferritin-like molecules in Pseudomonas aeruginosa: the product of the bfrA gene is a bacterial ferritin (FtnA) and not a bacterioferritin (Bfr). Yao H, Jepkorir G, Lovell S, Nama PV, Weeratunga S, Battaile KP, Rivera M Biochemistry 2011 Jun 14;50(23):5236-48 PubMed ID: 21574546