Pseudomonas aeruginosa PAO1, PA3546 (algX)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3546
Name
algX
Replicon chromosome
Genomic location 3971586 - 3973010 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252236.1
GI 15598742
Affymetrix PA3546_algX_at
Entrez 878551
GenBank AAG06934.1
INSDC AAG06934.1
NCBI Locus Tag PA3546
protein_id(GenBank) gb|AAG06934.1|AE004775_5|gnl|PseudoCAP|PA3546
TIGR NTL03PA03546
UniParc UPI00000C5A61
UniProtKB Acc Q51372
UniProtKB ID ALGX_PSEAE
UniRef100 UniRef100_Q51372
UniRef50 UniRef50_Q51372
UniRef90 UniRef90_Q51372

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate biosynthesis protein AlgX
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 3.02
Kyte-Doolittle Hydrophobicity Value -0.430
Molecular Weight (kDa) 52.6
Isoelectric Point (pI) 7.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4KNC SUGAR BINDING PROTEIN 05/09/13 Structural and functional characterization of Pseudomonas aeruginosa AlgX PSEUDOMONAS AERUGINOSA 2.141 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 12 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002120 (513 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3546
Search term: algX

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Alginate synthesis in Pseudomonas aeruginosa: the role of AlgL (alginate lyase) and AlgX.
Monday SR, Schiller NL
J. Bacteriol. 1996 Feb;178(3):625-32
PubMed ID: 8550492
AlgX is a periplasmic protein required for alginate biosynthesis in Pseudomonas aeruginosa.
Robles-Price A, Wong TY, Sletta H, Valla S, Schiller NL
J. Bacteriol. 2004 Nov;186(21):7369-77
PubMed ID: 15489449
Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX.
Weadge JT, Yip PP, Robinson H, Arnett K, Tipton PA, Howell PL
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2010 May;66(Pt 5):588-91
PubMed ID: 20445266