Pseudomonas aeruginosa PAO1, PA3649 (mucP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3649
Name
mucP
Synonym: rseP
Replicon chromosome
Genomic location 4087526 - 4088878 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252339.1
GI 15598845
Affymetrix PA3649_at
Entrez 880507
GenBank AAG07037.1
INSDC CEI78504.1
NCBI Locus Tag PA3649
protein_id(GenBank) gb|AAG07037.1|AE004785_1|gnl|PseudoCAP|PA3649
TIGR NTL03PA03649
UniParc UPI0000139266
UniProtKB Acc A5JIY6
UniProtKB Acc A5JIY6
UniProtKB ID Y3649_PSEAE
UniProtKB ID A5JIY6_PSEAI
UniRef100 UniRef100_Q9HXY3
UniRef50 UniRef50_Q9HXY3
UniRef90 UniRef90_Q9HXY3

Product

Feature Type CDS
Coding Frame 1
Product Name
MucP
Product Name Confidence: Class 4
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.33
Kyte-Doolittle Hydrophobicity Value 0.268
Molecular Weight (kDa) 48.5
Isoelectric Point (pI) 6.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB
Category Alginate regulation
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002025 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3649
Search term: mucP
Search term: MucP

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 66
Genomic Position 4087989
Position in ORF 464(1353)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 4480
Genomic Position 4087699
Position in ORF 174(1353)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_41_G6
Genomic Position 4088806
Position in ORF 1281(1353)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_17140
Mutant ID PA14NR: 55644
Genomic Position 1469153
Position in ORF 13(1353)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_17140
Mutant ID PA14NR: 38931
Genomic Position 1470101
Position in ORF 961(1353)
Reference 16477005

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity.
Damron FH, Yu HD
J. Bacteriol. 2011 Jan;193(1):286-91
PubMed ID: 21036998
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc. Natl. Acad. Sci. U.S.A. 2007 May;104(19):8107-12
PubMed ID: 17470813
Cell-surface signaling in Pseudomonas: stress responses, iron transport, and pathogenicity.
Llamas MA, Imperi F, Visca P, Lamont IL
FEMS Microbiol. Rev. 2014 Jul;38(4):569-97
PubMed ID: 24923658
Differential proteolysis of sigma regulators controls cell-surface signalling in Pseudomonas aeruginosa.
Draper RC, Martin LW, Beare PA, Lamont IL
Mol. Microbiol. 2011 Dec;82(6):1444-53
PubMed ID: 22040024