Pseudomonas aeruginosa PAO1, PA3649 (mucP)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: rseP
Replicon chromosome
Genomic location 4087526 - 4088878 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_252339.1
GI 15598845
Affymetrix PA3649_at
Entrez 880507
GenBank AAG07037.1
INSDC CEI78504.1
NCBI Locus Tag PA3649
protein_id(GenBank) gb|AAG07037.1|AE004785_1|gnl|PseudoCAP|PA3649
UniParc UPI0000139266
UniProtKB Acc A5JIY6
UniProtKB Acc A5JIY6
UniProtKB ID Y3649_PSEAE
UniRef100 UniRef100_Q9HXY3
UniRef50 UniRef50_Q9HXY3
UniRef90 UniRef90_Q9HXY3


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 4
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.33
Kyte-Doolittle Hydrophobicity Value 0.268
Molecular Weight (kDa) 48.5
Isoelectric Point (pI) 6.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB
Category Alginate regulation
Host Organism

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002025 (561 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3649
Search term: mucP
Search term: MucP

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 66
Genomic Position 4087989
Position in ORF 464(1353)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 4480
Genomic Position 4087699
Position in ORF 174(1353)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_41_G6
Genomic Position 4088806
Position in ORF 1281(1353)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_17140
Mutant ID PA14NR: 55644
Genomic Position 1469153
Position in ORF 13(1353)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_17140
Mutant ID PA14NR: 38931
Genomic Position 1470101
Position in ORF 961(1353)
Reference 16477005


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity.
Damron FH, Yu HD
J. Bacteriol. 2011 Jan;193(1):286-91
PubMed ID: 21036998
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc. Natl. Acad. Sci. U.S.A. 2007 May;104(19):8107-12
PubMed ID: 17470813
Cell-surface signaling in Pseudomonas: stress responses, iron transport, and pathogenicity.
Llamas MA, Imperi F, Visca P, Lamont IL
FEMS Microbiol. Rev. 2014 Jul;38(4):569-97
PubMed ID: 24923658
Differential proteolysis of sigma regulators controls cell-surface signalling in Pseudomonas aeruginosa.
Draper RC, Martin LW, Beare PA, Lamont IL
Mol. Microbiol. 2011 Dec;82(6):1444-53
PubMed ID: 22040024