Pseudomonas aeruginosa PAO1, PA3676 (mexK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3676
Name
mexK
Replicon chromosome
Genomic location 4116188 - 4119265 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_252366.1
GI 15598872
Affymetrix PA3676_at
Entrez 879602
GenBank AAG07064.1
INSDC AAG07064.1
NCBI Locus Tag PA3676
protein_id(GenBank) gb|AAG07064.1|AE004787_4|gnl|PseudoCAP|PA3676
TIGR NTL03PA03676
UniParc UPI00000C5AB4
UniProtKB Acc Q9HXW4
UniProtKB ID Q9HXW4_PSEAE
UniRef100 UniRef100_V6AB11
UniRef50 UniRef50_B7V2T7
UniRef90 UniRef90_B7V2T7

Product

Feature Type CDS
Coding Frame 1
Product Name
MexK
Product Name Confidence: Class 1
Synonyms probable RND efflux transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.84
Kyte-Doolittle Hydrophobicity Value 0.233
Molecular Weight (kDa) 113.3
Isoelectric Point (pI) 6.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexK MexK is the inner membrane resistance-nodulation-cell division (RND) transporter in the MexJK multidrug efflux protein. [PMID:12193619] ARO:3003693 protein homolog model Perfect 1949.5 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 486 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000156 (999 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3676
Search term: mexK
Search term: MexK

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The MexJK efflux pump of Pseudomonas aeruginosa requires OprM for antibiotic efflux but not for efflux of triclosan.
Chuanchuen R, Narasaki CT, Schweizer HP
J Bacteriol 2002 Sep;184(18):5036-44
PubMed ID: 12193619