Pseudomonas aeruginosa PAO1, PA3677 (mexJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3677
Name
mexJ
Replicon chromosome
Genomic location 4119270 - 4120373 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252367.1
GI 15598873
Affymetrix PA3677_at
Entrez 879603
GenBank AAG07065.1
INSDC AAG07065.1
NCBI Locus Tag PA3677
protein_id(GenBank) gb|AAG07065.1|AE004787_5|gnl|PseudoCAP|PA3677
TIGR NTL03PA03677
UniParc UPI00000C5AB5
UniProtKB Acc Q9HXW3
UniProtKB ID Q9HXW3_PSEAE
UniRef100 UniRef100_A0A069Q6H1
UniRef50 UniRef50_Q4KHJ5
UniRef90 UniRef90_W0YTY2

Product

Feature Type CDS
Coding Frame 1
Product Name
MexJ
Product Name Confidence: Class 1
Synonyms probable RND efflux membrane fusion protein precursor
Evidence for Translation
Charge (pH 7) 6.19
Kyte-Doolittle Hydrophobicity Value -0.238
Molecular Weight (kDa) 40.1
Isoelectric Point (pI) 9.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexJ MexJ is the membrane fusion protein of the MexJK multidrug efflux protein. [PMID:12193619] ARO:3003692 protein homolog model Perfect 697.2 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 443 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000154 (998 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3677
Search term: mexJ
Search term: MexJ

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The MexJK efflux pump of Pseudomonas aeruginosa requires OprM for antibiotic efflux but not for efflux of triclosan.
Chuanchuen R, Narasaki CT, Schweizer HP
J Bacteriol 2002 Sep;184(18):5036-44
PubMed ID: 12193619