Pseudomonas aeruginosa PAO1, PA3678 (mexL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3678
Name
mexL
Replicon chromosome
Genomic location 4120469 - 4121107 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252368.1
GI 15598874
Affymetrix PA3678_at
Entrez 880594
GenBank AAG07066.1
INSDC AAG07066.1
NCBI Locus Tag PA3678
protein_id(GenBank) gb|AAG07066.1|AE004787_6|gnl|PseudoCAP|PA3678
TIGR NTL03PA03678
UniParc UPI00000C5AB6
UniProtKB Acc Q9HXW2
UniProtKB ID Q9HXW2_PSEAE
UniRef100 UniRef100_W0YWF3
UniRef50 UniRef50_A6V1B0
UniRef90 UniRef90_A6V1B0

Product

Feature Type CDS
Coding Frame 1
Product Name
MexL
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.68
Kyte-Doolittle Hydrophobicity Value -0.117
Molecular Weight (kDa) 23.2
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexL MexL is a specific repressor of mexJK transcription and autoregulates its own expression. [PMID:15855505] ARO:3003710 protein homolog model Perfect 417.5 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 370 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001962 (826 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3678
Search term: mexL
Search term: MexL

Human Homologs

References

The MexJK efflux pump of Pseudomonas aeruginosa requires OprM for antibiotic efflux but not for efflux of triclosan.
Chuanchuen R, Narasaki CT, Schweizer HP
J Bacteriol 2002 Sep;184(18):5036-44
PubMed ID: 12193619
Molecular characterization of MexL, the transcriptional repressor of the mexJK multidrug efflux operon in Pseudomonas aeruginosa.
Chuanchuen R, Gaynor JB, Karkhoff-Schweizer R, Schweizer HP
Antimicrob Agents Chemother 2005 May;49(5):1844-51
PubMed ID: 15855505