Pseudomonas aeruginosa PAO1, PA3685 (yeaZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3685
Name
yeaZ
Synonym: tRNA threonylcarbamoyladenosine biosynthesis protein
Replicon chromosome
Genomic location 4126164 - 4126844 (- strand)

Cross-References

RefSeq NP_252375.1
GI 15598881
Affymetrix PA3685_at
DNASU PaCD00007614
Entrez 879145
GenBank AAG07073.1
INSDC AAG07073.1
NCBI Locus Tag PA3685
protein_id(GenBank) gb|AAG07073.1|AE004788_3|gnl|PseudoCAP|PA3685
TIGR NTL03PA03685
UniParc UPI00000C5ABC
UniProtKB Acc Q9HXV5
UniProtKB ID Q9HXV5_PSEAE
UniRef100 UniRef100_Q9HXV5
UniRef50 UniRef50_A4XXP3
UniRef90 UniRef90_W0YV43

Product

Feature Type CDS
Coding Frame 1
Product Name
YeaZ
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.10
Kyte-Doolittle Hydrophobicity Value 0.166
Molecular Weight (kDa) 24.2
Isoelectric Point (pI) 4.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5BR9 UNKNOWN FUNCTION 05/29/15 Crystal structure of an uncharacterized protein with similarity to peptidase YEAZ from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.35 X-RAY DIFFRACTION 100.0
4Y0W BIOSYNTHETIC PROTEIN 02/06/15 YeaZ from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO579 2.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 511 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001992 (561 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3685
Search term: yeaZ
Search term: YeaZ

References

Crystal structure of YeaZ from Pseudomonas aeruginosa.
Vecchietti D, Ferrara S, Rusmini R, Macchi R, Milani M, Bertoni G
Biochem. Biophys. Res. Commun. 2016 Feb;470(2):460-5
PubMed ID: 26768361
Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside.
Deutsch C, El Yacoubi B, de Crécy-Lagard V, Iwata-Reuyl D
J. Biol. Chem. 2012 Apr;287(17):13666-73
PubMed ID: 22378793