Pseudomonas aeruginosa PAO1, PA3713 (spdH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3713
Name
spdH
Synonym: spdH
Replicon chromosome
Genomic location 4158143 - 4160005 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentCleaved spermidine into 1, 3-diaminopropane and 4-aminobutyraldehyde. Hydrolyzed spermine into spermidine and 3-aminopropanaldehyde

Cross-References

RefSeq NP_252402.1
GI 15598908
Affymetrix PA3713_at
Entrez 880404
GenBank AAG07100.1
INSDC AAG07100.1
NCBI Locus Tag PA3713
protein_id(GenBank) gb|AAG07100.1|AE004790_5|gnl|PseudoCAP|PA3713
TIGR NTL03PA03713
UniParc UPI00000C5AD4
UniProtKB Acc Q9HXS8
UniProtKB ID Q9HXS8_PSEAE
UniRef100 UniRef100_W0YY94
UniRef50 UniRef50_K9NF77
UniRef90 UniRef90_W0YY94

Product

Feature Type CDS
Coding Frame 1
Product Name
spermidine dehydrogenase, SpdH
Product Name Confidence: Class 1
Synonyms spermidine dehydrogenase, SpdH
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -2.71
Kyte-Doolittle Hydrophobicity Value -0.315
Molecular Weight (kDa) 68.9
Isoelectric Point (pI) 6.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7D9F OXIDOREDUCTASE 10/13/20 SpdH Spermidine dehydrogenase SeMet Structure Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.85 X-RAY DIFFRACTION 100.0
7D9J OXIDOREDUCTASE 10/13/20 SpdH Spermidine dehydrogenase Y443A mutant Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.19 X-RAY DIFFRACTION 99.8
7D9I OXIDOREDUCTASE 10/13/20 SpdH Spermidine dehydrogenase D282A mutant Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.1 X-RAY DIFFRACTION 99.8
7D9G OXIDOREDUCTASE 10/13/20 SpdH Spermidine dehydrogenase native structure Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.4 X-RAY DIFFRACTION 100.0
7D9H OXIDOREDUCTASE 10/13/20 SpdH Spermidine dehydrogenase N33 truncation structure Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.31 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001969 (322 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3713
Search term: spdH

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization and a role of Pseudomonas aeruginosa spermidine dehydrogenase in polyamine catabolism.
Dasu VV, Nakada Y, Ohnishi-Kameyama M, Kimura K, Itoh Y
Microbiology (Reading) 2006 Aug;152(Pt 8):2265-2272
PubMed ID: 16849793
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994