Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3713
|
Name |
spdH
Synonym: spdH |
Replicon | chromosome |
Genomic location | 4158143 - 4160005 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Comment | Cleaved spermidine into 1, 3-diaminopropane and 4-aminobutyraldehyde. Hydrolyzed spermine into spermidine and 3-aminopropanaldehyde |
RefSeq | NP_252402.1 |
GI | 15598908 |
Affymetrix | PA3713_at |
Entrez | 880404 |
GenBank | AAG07100.1 |
INSDC | AAG07100.1 |
NCBI Locus Tag | PA3713 |
protein_id(GenBank) | gb|AAG07100.1|AE004790_5|gnl|PseudoCAP|PA3713 |
TIGR | NTL03PA03713 |
UniParc | UPI00000C5AD4 |
UniProtKB Acc | Q9HXS8 |
UniProtKB ID | Q9HXS8_PSEAE |
UniRef100 | UniRef100_W0YY94 |
UniRef50 | UniRef50_K9NF77 |
UniRef90 | UniRef90_W0YY94 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
spermidine dehydrogenase, SpdH
|
Synonyms |
spermidine dehydrogenase, SpdH |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -2.71 |
Kyte-Doolittle Hydrophobicity Value | -0.315 |
Molecular Weight (kDa) | 68.9 |
Isoelectric Point (pI) | 6.71 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7D9F | OXIDOREDUCTASE | 10/13/20 | SpdH Spermidine dehydrogenase SeMet Structure | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.85 | X-RAY DIFFRACTION | 100.0 |
7D9J | OXIDOREDUCTASE | 10/13/20 | SpdH Spermidine dehydrogenase Y443A mutant | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.19 | X-RAY DIFFRACTION | 99.8 |
7D9I | OXIDOREDUCTASE | 10/13/20 | SpdH Spermidine dehydrogenase D282A mutant | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.1 | X-RAY DIFFRACTION | 99.8 |
7D9G | OXIDOREDUCTASE | 10/13/20 | SpdH Spermidine dehydrogenase native structure | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.4 | X-RAY DIFFRACTION | 100.0 |
7D9H | OXIDOREDUCTASE | 10/13/20 | SpdH Spermidine dehydrogenase N33 truncation structure | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.31 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001969 (322 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3713
Search term: spdH
Search term: spermidine dehydrogenase, SpdH
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Characterization and a role of Pseudomonas aeruginosa spermidine dehydrogenase in polyamine catabolism.
Dasu VV, Nakada Y, Ohnishi-Kameyama M, Kimura K, Itoh Y
Microbiology (Reading) 2006 Aug;152(Pt 8):2265-2272
PubMed ID: 16849793
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|