Pseudomonas aeruginosa PAO1, PA3774

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3774
Name
Replicon	chromosome
Genomic location	4232258 - 4233400 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_252463.1
GI	15598969
Affymetrix	PA3774_at
Entrez	880599
GenBank	AAG07161.1
INSDC	AAG07161.1
NCBI Locus Tag	PA3774
protein_id(GenBank)	gb|AAG07161.1|AE004796_6|gnl|PseudoCAP|PA3774
TIGR	NTL03PA03774
UniParc	UPI00000C5B06
UniProtKB Acc	Q9HXM1
UniProtKB ID	Q9HXM1_PSEAE
UniRef100	UniRef100_S0J5Y1
UniRef50	UniRef50_S0ACH9
UniRef90	UniRef90_V6AAR1

Product

Feature Type	CDS
Coding Frame	1
Product Name	histone deacetylase-like amidohydrolase Product Name Confidence: Class 2
Synonyms	probable acetylpolyamine aminohydrolase
Evidence for Translation
Charge (pH 7)	-5.57
Kyte-Doolittle Hydrophobicity Value	-0.113
Molecular Weight (kDa)	41.0
Isoelectric Point (pI)	6.34

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
5G13	HYDROLASE	03/23/16	Pseudomonas aeruginosa HDAH (H143A) unliganded.	PSEUDOMONAS AERUGINOSA	1.99	X-RAY DIFFRACTION	99.7
5G0Y	HYDROLASE	03/23/16	Pseudomonas aeruginosa HDAH unliganded.	PSEUDOMONAS AERUGINOSA	2.29	X-RAY DIFFRACTION	100.0
5G10	HYDROLASE	03/23/16	Pseudomonas aeruginosa HDAH bound to 9,9,9 trifluoro-8,8-dihydroy-N-phenylnonanamide	PSEUDOMONAS AERUGINOSA	1.71	X-RAY DIFFRACTION	100.0
5G12	HYDROLASE	03/23/16	Pseudomonas aeruginosa HDAH (Y313F) unliganded.	PSEUDOMONAS AERUGINOSA	2.02	X-RAY DIFFRACTION	99.7
5LI3	SIGNALING PROTEIN	07/14/16	Crystal structure of HDAC-like protein from P. aeruginosa in complex with a photo-switchable inhibitor.	Pseudomonas aeruginosa	2.4	X-RAY DIFFRACTION	100.0
5G0X	HYDROLASE	03/23/16	Pseudomonas aeruginosa HDAH bound to acetate.	PSEUDOMONAS AERUGINOSA	1.7	X-RAY DIFFRACTION	100.0
5G11	HYDROLASE	03/23/16	Pseudomonas aeruginosa HDAH bound to PFSAHA.	PSEUDOMONAS AERUGINOSA	2.48	X-RAY DIFFRACTION	99.7

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 443 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001890 (353 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3774 Search term: histone deacetylase-like amidohydrolase

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000216271 histone deacetylase 10 [Source:HGNC Symbol;Acc:HGNC:18128] E-value: 1.7e-39 Percent Identity: 37.3

References

A fluorescence lifetime-based binding assay for acetylpolyamine amidohydrolases from Pseudomonas aeruginosa using a [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD) ligand probe. Meyners C, Wawrzinek R, Krämer A, Hinz S, Wessig P, Meyer-Almes FJ Anal Bioanal Chem 2014 Aug;406(20):4889-97 PubMed ID: 24871864

Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa. Krämer A, Herzer J, Overhage J, Meyer-Almes FJ BMC Biochem 2016 Mar 9;17:4 PubMed ID: 26956223