Pseudomonas aeruginosa PAO1, PA3774

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3774
Name
Replicon chromosome
Genomic location 4232258 - 4233400 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_252463.1
GI 15598969
Affymetrix PA3774_at
Entrez 880599
GenBank AAG07161.1
INSDC AAG07161.1
NCBI Locus Tag PA3774
protein_id(GenBank) gb|AAG07161.1|AE004796_6|gnl|PseudoCAP|PA3774
TIGR NTL03PA03774
UniParc UPI00000C5B06
UniProtKB Acc Q9HXM1
UniProtKB ID Q9HXM1_PSEAE
UniRef100 UniRef100_S0J5Y1
UniRef50 UniRef50_S0ACH9
UniRef90 UniRef90_V6AAR1

Product

Feature Type CDS
Coding Frame 1
Product Name
histone deacetylase-like amidohydrolase
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -5.57
Kyte-Doolittle Hydrophobicity Value -0.113
Molecular Weight (kDa) 41.0
Isoelectric Point (pI) 6.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5G11 HYDROLASE 03/23/16 Pseudomonas aeruginosa HDAH bound to PFSAHA. PSEUDOMONAS AERUGINOSA 2.48 X-RAY DIFFRACTION 99.7
5G0Y HYDROLASE 03/23/16 Pseudomonas aeruginosa HDAH unliganded. PSEUDOMONAS AERUGINOSA 2.29 X-RAY DIFFRACTION 100.0
5LI3 SIGNALING PROTEIN 07/14/16 Crystal structure of HDAC-like protein from P. aeruginosa in complex with a photo-switchable inhibitor. PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0
5G13 HYDROLASE 03/23/16 Pseudomonas aeruginosa HDAH (H143A) unliganded. PSEUDOMONAS AERUGINOSA 1.99 X-RAY DIFFRACTION 99.7
5G0X HYDROLASE 03/23/16 Pseudomonas aeruginosa HDAH bound to acetate. PSEUDOMONAS AERUGINOSA 1.7 X-RAY DIFFRACTION 100.0
5G10 HYDROLASE 03/23/16 Pseudomonas aeruginosa HDAH bound to 9,9,9 trifluoro-8,8-dihydroy-N-phenylnonanamide PSEUDOMONAS AERUGINOSA 1.71 X-RAY DIFFRACTION 100.0
5G12 HYDROLASE 03/23/16 Pseudomonas aeruginosa HDAH (Y313F) unliganded. PSEUDOMONAS AERUGINOSA 2.02 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 443 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001890 (360 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3774

Human Homologs

Ensembl 86, assembly GRCh38.p7
histone deacetylase 6 [Source:HGNC Symbol;Acc:HGNC:14064]
E-value: 1.4e-41
Percent Identity: 36.1

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_21_D6
Genomic Position 4233342
Position in ORF 1085(1143)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 37192
Genomic Position 4233094
Position in ORF 837(1143)
Reference 14617778

Transposon Insertions in Orthologs

References

A fluorescence lifetime-based binding assay for acetylpolyamine amidohydrolases from Pseudomonas aeruginosa using a [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD) ligand probe.
Meyners C, Wawrzinek R, Krämer A, Hinz S, Wessig P, Meyer-Almes FJ
Anal Bioanal Chem 2014 Aug;406(20):4889-97
PubMed ID: 24871864
Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa.
Krämer A, Herzer J, Overhage J, Meyer-Almes FJ
BMC Biochem. 2016 Mar;17
PubMed ID: 26956223