Pseudomonas aeruginosa PAO1, PA3803 (gcpE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3803
Name
gcpE
Replicon chromosome
Genomic location 4262378 - 4263493 (- strand)

Cross-References

RefSeq NP_252492.1
GI 15598998
Affymetrix PA3803_at
Entrez 879053
GenBank AAG07190.1
INSDC AAG07190.1
NCBI Locus Tag PA3803
protein_id(GenBank) gb|AAG07190.1|AE004798_13|gnl|PseudoCAP|PA3803
TIGR NTL03PA03803
UniParc UPI000012D998
UniProtKB Acc Q9HXJ4
UniProtKB ID ISPG_PSEAE
UniRef100 UniRef100_B7UWI8
UniRef50 UniRef50_P72241
UniRef90 UniRef90_B7UWI8

Product

Feature Type CDS
Coding Frame 1
Product Name
probable isoprenoid biosynthetic protein GcpE
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.45
Kyte-Doolittle Hydrophobicity Value -0.049
Molecular Weight (kDa) 40.1
Isoelectric Point (pI) 6.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 515 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001863 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3803
Search term: gcpE

Human Homologs

References

GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli.
Altincicek B, Kollas AK, Sanderbrand S, Wiesner J, Hintz M, Beck E, Jomaa H
J Bacteriol 2001 Apr;183(8):2411-6
PubMed ID: 11274098
Primary structure of histidine-tRNA synthetase and characterization of hisS transcripts.
Freedman R, Gibson B, Donovan D, Biemann K, Eisenbeis S, Parker J, Schimmel P
J Biol Chem 1985 Aug 25;260(18):10063-8
PubMed ID: 2991272
Sequence and characterization of the gcpE gene of Escherichia coli.
Baker J, Franklin DB, Parker J
FEMS Microbiol Lett 1992 Jul 1;73(1-2):175-80
PubMed ID: 1521767