Pseudomonas aeruginosa PAO1, PA3861 (rhl)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3861
Name
rhl
Replicon chromosome
Genomic location 4321104 - 4322627 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentTranslation confirmed using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer. Coordinates verified using RT-PCR (PMID:24291602).

Cross-References

RefSeq NP_252550.3
GI 161486761
Affymetrix PA3861_rhlB_at
Entrez 879804
GenBank AAG07248.1
INSDC AAG07248.1
NCBI Locus Tag PA3861
protein_id(GenBank) gb|AAG07248.1|AE004803_4|gnl|PseudoCAP|PA3861
TIGR NTL03PA03861
UniParc UPI00000C5B49
UniProtKB Acc Q9HXE5
UniProtKB ID RHLB_PSEAE
UniRef100 UniRef100_B7UUT0
UniRef50 UniRef50_Q887N8
UniRef90 UniRef90_B7UUT0

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP-dependent RNA helicase RhlB
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.43
Kyte-Doolittle Hydrophobicity Value -0.213
Molecular Weight (kDa) 44.3
Isoelectric Point (pI) 6.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 628 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001808 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3861
Search term: rhl

Human Homologs

Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 3 X-linked [Source:HGNC Symbol;Acc:HGNC:2745]
E-value: 4.0e-57
Percent Identity: 38.3

References

A DEAD-box RNA helicase in the Escherichia coli RNA degradosome.
Py B, Higgins CF, Krisch HM, Carpousis AJ
Nature 1996 May 9;381(6578):169-72
PubMed ID: 8610017
LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan 16;96:328-342
PubMed ID: 24291602