Pseudomonas aeruginosa PAO1, PA3878 (narX)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 4345010 - 4346878 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_252567.1
GI 15599073
Affymetrix PA3878_narX_at
Entrez 878321
GenBank AAG07265.1
INSDC AAG07265.1
NCBI Locus Tag PA3878
protein_id(GenBank) gb|AAG07265.1|AE004805_3|gnl|PseudoCAP|PA3878
UniParc UPI00000D426E
UniProtKB Acc G3XD66
UniRef100 UniRef100_W0YVS9
UniRef50 UniRef50_Q02S43
UniRef90 UniRef90_Q02S43


Feature Type CDS
Coding Frame 1
Product Name
two-component sensor NarX
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (69.32 kDa, PMID:21751344)
Charge (pH 7) -9.31
Kyte-Doolittle Hydrophobicity Value -0.309
Molecular Weight (kDa) 69.3
Isoelectric Point (pI) 5.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 415 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001793 (389 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3878
Search term: narX

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 3104
Genomic Position 4345534
Position in ORF 525(1869)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 42490
Genomic Position 4346256
Position in ORF 1247(1869)
Reference 14617778

Transposon Insertions in Orthologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Structure of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.
Stewart V, Parales J, Merkel SM
J. Bacteriol. 1989 Apr;171(4):2229-34
PubMed ID: 2649492
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
The OpdQ porin of Pseudomonas aeruginosa is regulated by environmental signals associated with cystic fibrosis including nitrate-induced regulation involving the NarXL two-component system.
Fowler RC, Hanson ND
Microbiologyopen 2015 Dec;4(6):967-82
PubMed ID: 26459101