Pseudomonas aeruginosa PAO1, PA3946 (rocS1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3946
Name
rocS1
Synonym: rocS1
Replicon chromosome
Genomic location 4423212 - 4426850 (- strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs
CommentOverexpression of rocS1 leads to the production of CupB and CupC fimbriae, as well as repression of the MexAB-OprM efflux pump. RocS1 can interact with three response regulators: RocA2 (PA3045), RocA1 (PA3948) and RocR (PA3947).

Cross-References

RefSeq NP_252635.1
GI 15599141
Affymetrix PA3946_at
Entrez 878870
GenBank AAG07333.1
INSDC AAG07333.1
NCBI Locus Tag PA3946
protein_id(GenBank) gb|AAG07333.1|AE004812_8|gnl|PseudoCAP|PA3946
TIGR NTL03PA03946
UniParc UPI00000C5B8E
UniProtKB Acc Q9HX70
UniProtKB ID Q9HX70_PSEAE
UniRef100 UniRef100_Q9HX70
UniRef50 UniRef50_W0WA70
UniRef90 UniRef90_W0WA70

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component sensor RocS1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -26.29
Kyte-Doolittle Hydrophobicity Value -0.058
Molecular Weight (kDa) 134.7
Isoelectric Point (pI) 5.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001586 (824 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3946
Search term: rocS1

Human Homologs

References

A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes.
Kulasekara HD, Ventre I, Kulasekara BR, Lazdunski A, Filloux A, Lory S
Mol Microbiol 2005 Jan;55(2):368-80
PubMed ID: 15659157
Assembly of fimbrial structures in Pseudomonas aeruginosa: functionality and specificity of chaperone-usher machineries.
Ruer S, Stender S, Filloux A, de Bentzmann S
J Bacteriol 2007 May;189(9):3547-55
PubMed ID: 17293418
Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression.
Sivaneson M, Mikkelsen H, Ventre I, Bordi C, Filloux A
Mol Microbiol 2011 Mar;79(5):1353-66
PubMed ID: 21205015