Pseudomonas aeruginosa PAO1, PA3977 (hemL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3977
Name
hemL
Replicon chromosome
Genomic location 4457362 - 4458645 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252666.1
GI 15599172
Affymetrix PA3977_hemL_at
Entrez 880859
GenBank AAG07364.1
INSDC AAG07364.1
NCBI Locus Tag PA3977
protein_id(GenBank) gb|AAG07364.1|AE004815_8|gnl|PseudoCAP|PA3977
TIGR NTL03PA03977
UniParc UPI000012BB8E
UniProtKB Acc P48247
UniProtKB ID GSA_PSEAE
UniRef100 UniRef100_B7V9D9
UniRef50 UniRef50_Q8X4V5
UniRef90 UniRef90_Q4ZNA0

Product

Feature Type CDS
Coding Frame 1
Product Name
glutamate-1-semialdehyde 2,1-aminomutase
Product Name Confidence: Class 1
Synonyms glutamate-1-semialdehyde aminotransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -9.26
Kyte-Doolittle Hydrophobicity Value 0.099
Molecular Weight (kDa) 45.4
Isoelectric Point (pI) 5.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5I92 ISOMERASE 02/19/16 Crystal structure of Glutamate-1-semialdehyde 2,1- aminomutase (GSA) from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.75 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 638 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001704 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3977
Search term: hemL

Human Homologs

References

Cloning, mapping and characterization of the Pseudomonas aeruginosa hemL gene.
Hungerer C, Troup B, Römling U, Jahn D
Mol Gen Genet 1995 Aug 21;248(3):375-80
PubMed ID: 7565600
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994